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RT-PCR Related Discussions
  1. What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
  2. Gene Expression level of apoptotic-related genes by qReal Time RT PCR - (reply: 1)
  3. -RT works better than +RT? - (reply: 9)
  4. RT-PCR works but not consistent - (reply: 3)
  5. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 3)
  6. RT-PCR GAPDH Variability - (reply: 1)
  7. how to optimize rt-pcr for gene expression analysis - (reply: 3)
  8. how much cDNA do you use for RT-PCR? - (reply: 2)
  9. RT PCR of viral RNA segment - (reply: 1)
  10. wierd rt-pcr - (reply: 1)
  11. Transcript variation - measuring by RT-PCR - (reply: 3)
  12. When is the right time to adjust RNA concentrations for Rt-PCR? - (reply: 2)
  13. Inconsistent Real Time results- RT efficiency, high Ct values - (reply: 3)
  14. RT-PCR inconsistant results - (reply: 1)
  15. RT-PCR reagents KIT 1 or 2-step? - (reply: 3)
  16. Choice of one-step or two-steps RT-PCR - (reply: 3)
  17. Checking mRNA expression by RT-PCR - (reply: 2)
  18. quadraplex RT-PCR error - (reply: 1)
  19. Can a 96 well plate for rt PCR be used for ELISA? - 96 well plate , ELISA (reply: 2)
  20. RT-PCR from VERY limited quantity of tissue sample - Need suggestion on RNA isolation and RT kits to maximize cDNA yield (reply: 1)
  21. Failed RT-PCR amplification of 3-&5''-ligated small RNAs - (reply: 5)
  22. cDNA quantification by non-PCR method - measuring RT efficiency (reply: 1)
  23. Help -designing primers for real time RT-PCR - (reply: 1)
  24. help with RT-PCR - DNase usage - (reply: 2)
  25. Getting primer dimers in RT-PCR on and off? - (reply: 2)
  26. What are different ways of genotyping a transgenic mouse? - what is best way to do genotyping--PCR, qRT-PCR or RT-PCR (reply: 3)
  27. 1:10 serial dilution in rt-pcr with slope of -0.58 ... - (reply: 1)
  28. RT-PCR or disulfite treatment, which is better? - (reply: 5)
  29. RNA extraction from laser capture microdissections - how to see low expressed genes from trought RT/qPCR on laser capture m (reply: 2)
  30. Housekeeping genes for RT-PCR in soybean - (reply: 6)
  31. Stem-loop RT primers: how to anneal? - (reply: 1)
  32. RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? - (reply: 1)
  33. Primers from RT-PCR database - RT-PCRdatabase primers giving primer dimers (reply: 1)
  34. Troubleshooting RT-PCR - (reply: 7)
  35. Can I run q RT real-time PCR based on cDNA amount? - (reply: 1)
  36. antibody incubation on western blots - difference between 4°C and RT (reply: 10)
  37. Statistics: real-time RT-PCR - (reply: 4)
  38. RT-Pcr without RNA isolation? - (reply: 3)
  39. Determining the RT concentration in HIV-1 samples through Sandwich ELISA - Not seeing any infection/RT activity/indication of virus (reply: 7)
  40. What is the suitable product size for conventional RT-PCR analysis - products with size over 1 kb? (reply: 6)
  41. puzzling RT question - (reply: 4)
  42. RT-PCR - necessary to run electrophoresis gels? - (reply: 4)
  43. RT PCR Primer dimer tissue dependent SYBR - (reply: 1)
  44. any problems with RT-PCR for large amplicon sizes? - very strange amplification curves (reply: 2)
  45. problem rt pcr long gene - (reply: 4)
  46. RT PCR produces nice crisp band but of wrong length :-) - (reply: 2)
  47. RT-PCR: Band in no reverse transcriptase lane - (reply: 18)
  48. Keep RT mix on ice for 4 hrs - RT-PCR (reply: 3)
  49. 2-step RT-PCR - (reply: 1)
  50. RT-PCR Controls (no DNA) show weird amplification - Complicated problem- urgent help needed! (reply: 4)
  51. Reverse Transcriptase PCR - Step by step protocol (reply: 6)
  52. About Reverse transcriptase PCR followed by PCR - primers (reply: 1)
  53. RT PCR - NO PCR PRODUCT (reply: 3)
  54. RNA quantification for Real Time RT-PCR - (reply: 2)
  55. Does non-digested RNA in cDNA sample have an effect on RT-PCR quantification? - (reply: 2)
  56. multiplex rt pcr - (reply: 5)
  57. minimum amount of rna in RT-PCR - (reply: 1)
  58. oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit? (reply: 2)
  59. improve the DNA cons. in RT-PCR - (reply: 2)
  60. ask for help about the RT-PCR - does different aoumnt of RNA affect the RT efficiency? (reply: 1)
  61. Representing RT-PCR Data - (reply: 2)
  62. RT-PCR kit - (reply: 3)
  63. Amplified Minus RT - (reply: 1)
  64. pcr product afetr reverse transcriptase reaction - 4 bands i have not included the band which i want??? (reply: 5)
  65. Problem - same ct values in my RT positive and negative samples - (reply: 3)
  66. DNase treatment of RNA prior to RT-PCR - (reply: 4)
  67. one Step RT with Sybr and standard curve - (reply: 1)
  68. can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
  69. primer selection for RT prior to real time - an insiders hint!!! (reply: 1)
  70. loading control of RT-PCR - (reply: 3)
  71. RT-PCR - not qPCR! urgent please - how to choose primers (reply: 3)
  72. RT control product appears only 5 cycles away from unknown samples - (reply: 2)
  73. rt-pcr data and western blot data not compatible - what could be the explanation (reply: 5)
  74. RT PCR Primer - Software (reply: 2)
  75. RT-PCR primer design - (reply: 1)
  76. Problem with cloning p53-suggestions for RT-PCR - (reply: 1)
  77. oligo(dT) plus random primers in RT - (reply: 4)
  78. Relative Quantification in real-time RT-PCR - (reply: 5)
  79. No RT control showed strange results - (reply: 3)
  80. NTC's in RT or just qPCR? - (reply: 3)
  81. faster way to identify RNA virus other than RT-PCR? - (reply: 2)
  82. Two bands on RT-PCR product - (reply: 8)
  83. ASAP: ONE STEP Real time RT PCR kit for TWO step RT-PCR method? - (reply: 1)
  84. choice of reverse transcriptase - (reply: 4)
  85. Best product size for real-time RT-PCR - (reply: 4)
  86. Viral RT PCR - (reply: 2)
  87. RT-PCR machine/setup problems - (reply: 2)
  88. RT-PCR question - genomic DNA amplified with RNA (reply: 9)
  89. Long RT for 4.5 kb cDNA - (reply: 5)
  90. RNA storing/RT PCR using oligo dT - (reply: 5)
  91. HIV RT PCR KIT - (reply: 1)
  92. Housekeeping gene for RT-PCR on plant - RT PCR (reply: 1)
  93. RT-PCR primer design at 3'-UTR or ORF? - (reply: 2)
  94. RT PCR - (reply: 1)
  95. RT-PCR primers for E. coli LacZ gene - (reply: 3)
  96. Picking stable clones by RT-PCR.. - (reply: 2)
  97. RT-PCR with an old DNaseI? - (reply: 3)
  98. How can I get the 4.5kb Human typeI procollagen alpha1 chain cDNA Via RT-PCR fr - Seeking Help (reply: 14)
  99. RT-PCR on plasmid - (reply: 1)
  100. RT-PCR detection limits - (reply: 1)
  101. Cloning using RT-PCR for long fragment of 6kb - (reply: 1)
  102. Does Taq have reverse transcriptase activity? - (reply: 20)
  103. RT-PCR for large fragment - (reply: 7)
  104. RT-PCR problem - negative control (reply: 1)
  105. RT-PCR scale-down makes more sensitive? - (reply: 3)
  106. how much total RNA do you use for RT-PCR - (reply: 6)
  107. What kind of statistic methods? - RT-PCR (reply: 2)
  108. Internal Control RNA & RT-PCR - (reply: 1)
  109. RT-PCR some questions - (reply: 2)
  110. internal control in semi quantitative rt pcr - question (reply: 2)
  111. Storage of RT-PCR products - (reply: 2)
  112. real-time RT-PCR one cell - (reply: 2)
  113. RT-PCR and RE cut site-will this work? - (reply: 3)
  114. cDNA synthesis - which transcriptase is better? - (reply: 2)
  115. 3 bands on RT-PCR - (reply: 4)
  116. RT- PCR? - (reply: 2)
  117. High fidelity RT-PCR enzymes - looking for choices... (reply: 5)
  118. realtime RT-PCR/internal control - (reply: 3)
  119. RT-PCR gone bad - (reply: 4)
  120. how to produce a high-quality cDNA template for RT-PCR - produce a high-quality cDNA template (reply: 4)
  121. Why is DTT used in reverse transcription (RT) reaction? - (reply: 9)
  122. RT-PCR - (reply: 4)
  123. RT-PCR with B-cells as template - (reply: 1)
  124. primer design: end-point vs. RT - (reply: 1)
  125. RT problem !! - (reply: 1)
  126. PCR or RT-PCR? - (reply: 3)
  127. RT PCR - dilution prior to PCR (reply: 2)
  128. RT-PCR, why two PCR steps? - two PCRs after reverse transcription?? (reply: 2)
  129. Trouble shooting RT-PCR - (reply: 4)
  130. Which gene is the best internal control for RT-PCR - (reply: 5)
  131. RT-PCR;Northern-blotting - internal standard (reply: 4)
  132. RT-PCR, RNA isolation, etc. - (reply: 1)
  133. RT-PCR primer design guide - How to check gene structure and design the primer? (reply: 15)
  134. Detection in SmartCycler RT-PCR - (reply: 1)
  135. Help! DNA contamination in RNA and RT-PCR. - (reply: 1)
  136. Primer design - Primer design for RT-PCR (reply: 1)
  137. RT-PCR - (reply: 2)
  138. RT-PCR - (reply: 1)
  139. RT PCR - (reply: 1)
  140. RT-PCR, help needed - (reply: 2)
  141. RNA isolation from isolatedislets and RT-PCR - (reply: 1)
  142. problem with Rt-PCR - (reply: 1)
  143. C-MYC AND CYCLIN D1  / RT-PCR - (reply: 2)