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RT-PCR Related Discussions
What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs
(reply: 1)
Gene Expression level of apoptotic-related genes by qReal Time RT PCR -
(reply: 1)
-RT works better than +RT? -
(reply: 9)
RT-PCR works but not consistent -
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RT-PCR primer design - RT-PCR primer design...need ur help
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RT-PCR GAPDH Variability -
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how to optimize rt-pcr for gene expression analysis -
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how much cDNA do you use for RT-PCR? -
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RT PCR of viral RNA segment -
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wierd rt-pcr -
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Transcript variation - measuring by RT-PCR -
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When is the right time to adjust RNA concentrations for Rt-PCR? -
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Inconsistent Real Time results- RT efficiency, high Ct values -
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RT-PCR inconsistant results -
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RT-PCR reagents KIT 1 or 2-step? -
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Choice of one-step or two-steps RT-PCR -
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Checking mRNA expression by RT-PCR -
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quadraplex RT-PCR error -
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Can a 96 well plate for rt PCR be used for ELISA? - 96 well plate , ELISA
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RT-PCR from VERY limited quantity of tissue sample - Need suggestion on RNA isolation and RT kits to maximize cDNA yield
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Failed RT-PCR amplification of 3-&5''-ligated small RNAs -
(reply: 5)
cDNA quantification by non-PCR method - measuring RT efficiency
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Help -designing primers for real time RT-PCR -
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help with RT-PCR - DNase usage -
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Getting primer dimers in RT-PCR on and off? -
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What are different ways of genotyping a transgenic mouse? - what is best way to do genotyping--PCR, qRT-PCR or RT-PCR
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1:10 serial dilution in rt-pcr with slope of -0.58 ... -
(reply: 1)
RT-PCR or disulfite treatment, which is better? -
(reply: 5)
RNA extraction from laser capture microdissections - how to see low expressed genes from trought RT/qPCR on laser capture m
(reply: 2)
Housekeeping genes for RT-PCR in soybean -
(reply: 6)
Stem-loop RT primers: how to anneal? -
(reply: 1)
RT-PCR cloning: oligo dT-primed cDNA synthesis or anchored? -
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Primers from RT-PCR database - RT-PCRdatabase primers giving primer dimers
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Troubleshooting RT-PCR -
(reply: 7)
Can I run q RT real-time PCR based on cDNA amount? -
(reply: 1)
antibody incubation on western blots - difference between 4°C and RT
(reply: 10)
Statistics: real-time RT-PCR -
(reply: 4)
RT-Pcr without RNA isolation? -
(reply: 3)
Determining the RT concentration in HIV-1 samples through Sandwich ELISA - Not seeing any infection/RT activity/indication of virus
(reply: 7)
What is the suitable product size for conventional RT-PCR analysis - products with size over 1 kb?
(reply: 6)
puzzling RT question -
(reply: 4)
RT-PCR - necessary to run electrophoresis gels? -
(reply: 4)
RT PCR Primer dimer tissue dependent SYBR -
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any problems with RT-PCR for large amplicon sizes? - very strange amplification curves
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problem rt pcr long gene -
(reply: 4)
RT PCR produces nice crisp band but of wrong length :-) -
(reply: 2)
RT-PCR: Band in no reverse transcriptase lane -
(reply: 18)
Keep RT mix on ice for 4 hrs - RT-PCR
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2-step RT-PCR -
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RT-PCR Controls (no DNA) show weird amplification - Complicated problem- urgent help needed!
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Reverse Transcriptase PCR - Step by step protocol
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About Reverse transcriptase PCR followed by PCR - primers
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RT PCR - NO PCR PRODUCT
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RNA quantification for Real Time RT-PCR -
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Does non-digested RNA in cDNA sample have an effect on RT-PCR quantification? -
(reply: 2)
multiplex rt pcr -
(reply: 5)
minimum amount of rna in RT-PCR -
(reply: 1)
oligos as synthetic "miRNA" for quantification? - would an oligo / primer work in AB RT kit?
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improve the DNA cons. in RT-PCR -
(reply: 2)
ask for help about the RT-PCR - does different aoumnt of RNA affect the RT efficiency?
(reply: 1)
Representing RT-PCR Data -
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RT-PCR kit -
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Amplified Minus RT -
(reply: 1)
pcr product afetr reverse transcriptase reaction - 4 bands i have not included the band which i want???
(reply: 5)
Problem - same ct values in my RT positive and negative samples -
(reply: 3)
DNase treatment of RNA prior to RT-PCR -
(reply: 4)
one Step RT with Sybr and standard curve -
(reply: 1)
can Primers for RT-PCR be used Real-Time PCR directly? -
(reply: 2)
primer selection for RT prior to real time - an insiders hint!!!
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loading control of RT-PCR -
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RT-PCR - not qPCR! urgent please - how to choose primers
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RT control product appears only 5 cycles away from unknown samples -
(reply: 2)
rt-pcr data and western blot data not compatible - what could be the explanation
(reply: 5)
RT PCR Primer - Software
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RT-PCR primer design -
(reply: 1)
Problem with cloning p53-suggestions for RT-PCR -
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oligo(dT) plus random primers in RT -
(reply: 4)
Relative Quantification in real-time RT-PCR -
(reply: 5)
No RT control showed strange results -
(reply: 3)
NTC's in RT or just qPCR? -
(reply: 3)
faster way to identify RNA virus other than RT-PCR? -
(reply: 2)
Two bands on RT-PCR product -
(reply: 8)
ASAP: ONE STEP Real time RT PCR kit for TWO step RT-PCR method? -
(reply: 1)
choice of reverse transcriptase -
(reply: 4)
Best product size for real-time RT-PCR -
(reply: 4)
Viral RT PCR -
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RT-PCR machine/setup problems -
(reply: 2)
RT-PCR question - genomic DNA amplified with RNA
(reply: 9)
Long RT for 4.5 kb cDNA -
(reply: 5)
RNA storing/RT PCR using oligo dT -
(reply: 5)
HIV RT PCR KIT -
(reply: 1)
Housekeeping gene for RT-PCR on plant - RT PCR
(reply: 1)
RT-PCR primer design at 3'-UTR or ORF? -
(reply: 2)
RT PCR -
(reply: 1)
RT-PCR primers for E. coli LacZ gene -
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Picking stable clones by RT-PCR.. -
(reply: 2)
RT-PCR with an old DNaseI? -
(reply: 3)
How can I get the 4.5kb Human typeI procollagen alpha1 chain cDNA Via RT-PCR fr - Seeking Help
(reply: 14)
RT-PCR on plasmid -
(reply: 1)
RT-PCR detection limits -
(reply: 1)
Cloning using RT-PCR for long fragment of 6kb -
(reply: 1)
Does Taq have reverse transcriptase activity? -
(reply: 20)
RT-PCR for large fragment -
(reply: 7)
RT-PCR problem - negative control
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RT-PCR scale-down makes more sensitive? -
(reply: 3)
how much total RNA do you use for RT-PCR -
(reply: 6)
What kind of statistic methods? - RT-PCR
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Internal Control RNA & RT-PCR -
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RT-PCR some questions -
(reply: 2)
internal control in semi quantitative rt pcr - question
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Storage of RT-PCR products -
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real-time RT-PCR one cell -
(reply: 2)
RT-PCR and RE cut site-will this work? -
(reply: 3)
cDNA synthesis - which transcriptase is better? -
(reply: 2)
3 bands on RT-PCR -
(reply: 4)
RT- PCR? -
(reply: 2)
High fidelity RT-PCR enzymes - looking for choices...
(reply: 5)
realtime RT-PCR/internal control -
(reply: 3)
RT-PCR gone bad -
(reply: 4)
how to produce a high-quality cDNA template for RT-PCR - produce a high-quality cDNA template
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Why is DTT used in reverse transcription (RT) reaction? -
(reply: 9)
RT-PCR -
(reply: 4)
RT-PCR with B-cells as template -
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primer design: end-point vs. RT -
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RT problem !! -
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PCR or RT-PCR? -
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RT PCR - dilution prior to PCR
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RT-PCR, why two PCR steps? - two PCRs after reverse transcription??
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Trouble shooting RT-PCR -
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Which gene is the best internal control for RT-PCR -
(reply: 5)
RT-PCR;Northern-blotting - internal standard
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RT-PCR, RNA isolation, etc. -
(reply: 1)
RT-PCR primer design guide - How to check gene structure and design the primer?
(reply: 15)
Detection in SmartCycler RT-PCR -
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Help! DNA contamination in RNA and RT-PCR. -
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Primer design - Primer design for RT-PCR
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RT-PCR -
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RT-PCR -
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RT PCR -
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RT-PCR, help needed -
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RNA isolation from isolatedislets and RT-PCR -
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problem with Rt-PCR -
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C-MYC AND CYCLIN D1 / RT-PCR -
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