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Phosphatase Related Discussions
  1. Phosphatase treatment as a control for Phospho antibodies - (reply: 1)
  2. Mammalian Phosphatase inhibitors - (reply: 6)
  3. phophorylating PCR product after CIP - (reply: 2)
  4. dephos with alkaline phosphatase - (reply: 2)
  5. SAP incubation time - how long do you incubate for?? (reply: 1)
  6. cloning, ligation, dephosphorylation - ligation, dephosphorylation (reply: 3)
  7. Overtreatment by dephosphorylating enzymes - (reply: 7)
  8. Alkaline Phosphatase and PBS with Magnesium - (reply: 2)
  9. Sticky-end dephosphorylation - (reply: 3)
  10. Problem in dephosphorylation - (reply: 7)
  11. Dephosphorylation - (reply: 4)
  12. CIP of Vector and Kinase of Insert? - Is it necessary in blunt end cloning? (reply: 1)
  13. phosphatase inhibitor cocktails... - do you use them or a preffered one? (reply: 4)
  14. Phosphatase Inhibitors into IP lysis buffer! - (reply: 2)
  15. CIP and alkaline phosphatase - what's the difference? (reply: 5)
  16. interactin between alkaline phosphatase buffer and PNK buffer. - help please (reply: 1)
  17. Dephosphorylation nick problem - (reply: 2)
  18. phosphatase removal in ligation - (reply: 3)
  19. Troubles with vector dephosphorylation - troubles with vector dephosphorylation (reply: 2)
  20. Do I need CIP before my ligation - (reply: 4)
  21. CIP with restriction digestion - (reply: 5)
  22. protein dephosphorylation by alkaline phosphatase? - (reply: 8)
  23. Alkaline phosphatase cnjugated antibody - secondary antibody (reply: 5)
  24. phosphatase alkaline before cloning? - (reply: 3)
  25. Alkaline Phosphatase quantitation - Need help using nitrophenol (reply: 1)
  26. CIPping after Klenow blunting - Is CIP necessary in Blunt end ligation? (reply: 2)
  27. Rephosphorylation of SAP treated DNA fragment - (reply: 1)
  28. 3' overhang dephosphorylation? - dephosphorylation problems after KpnI dig (reply: 4)
  29. phosphatase inhibitors - what do I use for with ant-P-Ser IP? (reply: 1)
  30. dephosphorylation - (reply: 1)
  31. CIP problems - (reply: 1)