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1. mysterious thing! - (reply: 2)
2. troubles with small proteins (less than 20) on SDS page - (reply: 2)
3. stripping buffer! - didn't strip after 30min (reply: 3)
4. protein spreading in gel - (reply: 1)
5. PVDF and Nitrocellulose have different current V/mAmp! - why am I seeing less transfer while using PVDF? (reply: 3)
6. Tris Hcl running gel buffer problems - (reply: 6)
7. transfer problem - blue nitrocellulose paper (reply: 3)
8. sds-gel problem - (reply: 2)
9. SDS-PAGE problem - no bands around 20KDa (reply: 3)
10. Marker for Sample from Nuclear Extraction - (reply: 2)
11. Gels waiting for 90 min before transfer? - (reply: 6)
12. optimal conditions for wet transfer in western blotting - (reply: 3)
13. Laemmli buffer recipe - (reply: 3)
14. separation of loading buffer - (reply: 1)
15. zymogram of pectinase - activity staining of pectinase (reply: 2)
16. Immunoprecipitation - Immunoprecipitation protocol to obtain native immunoprecipitated prots (reply: 3)
17. western blot, I have a signal without primary antibody - (reply: 6)
18. transfer of high molecular weight proteins - (reply: 5)
19. Quench HRP with sodium azide - (reply: 3)
20. recipe for LDS based sample buffer - (reply: 1)
21. bubbles and overheating of western blot - (reply: 2)
22. Non-specific bands with secondary Antibody on Western Blotting - (reply: 1)
23. band extraction from sds page - (reply: 1)
24. Same samples but different results! - (reply: 3)
25. phosphoSer lower on films - (reply: 2)
26. Western blot control - Suggestions? (reply: 3)
27. Loading control for tears WB - GAPDH? (reply: 4)
28. western blot from heart lysates - membrane extractions too (reply: 2)
29. Problems with blot - (reply: 2)
30. protein concentration problem - (reply: 2)
2. troubles with small proteins (less than 20) on SDS page - (reply: 2)
3. stripping buffer! - didn't strip after 30min (reply: 3)
4. protein spreading in gel - (reply: 1)
5. PVDF and Nitrocellulose have different current V/mAmp! - why am I seeing less transfer while using PVDF? (reply: 3)
6. Tris Hcl running gel buffer problems - (reply: 6)
7. transfer problem - blue nitrocellulose paper (reply: 3)
8. sds-gel problem - (reply: 2)
9. SDS-PAGE problem - no bands around 20KDa (reply: 3)
10. Marker for Sample from Nuclear Extraction - (reply: 2)
11. Gels waiting for 90 min before transfer? - (reply: 6)
12. optimal conditions for wet transfer in western blotting - (reply: 3)
13. Laemmli buffer recipe - (reply: 3)
14. separation of loading buffer - (reply: 1)
15. zymogram of pectinase - activity staining of pectinase (reply: 2)
16. Immunoprecipitation - Immunoprecipitation protocol to obtain native immunoprecipitated prots (reply: 3)
17. western blot, I have a signal without primary antibody - (reply: 6)
18. transfer of high molecular weight proteins - (reply: 5)
19. Quench HRP with sodium azide - (reply: 3)
20. recipe for LDS based sample buffer - (reply: 1)
21. bubbles and overheating of western blot - (reply: 2)
22. Non-specific bands with secondary Antibody on Western Blotting - (reply: 1)
23. band extraction from sds page - (reply: 1)
24. Same samples but different results! - (reply: 3)
25. phosphoSer lower on films - (reply: 2)
26. Western blot control - Suggestions? (reply: 3)
27. Loading control for tears WB - GAPDH? (reply: 4)
28. western blot from heart lysates - membrane extractions too (reply: 2)
29. Problems with blot - (reply: 2)
30. protein concentration problem - (reply: 2)