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1. Immunstaining cells fixed in 10% formalin- possible? - (reply: 6)
2. Signal in the citoplasm instead of in the nucleus - (reply: 1)
3. Is it possible to preserve polylisine coated glasses? - (reply: 1)
4. Immunohistochemistry--How to quantify it? - (reply: 2)
5. Griess Assay reagents - Expiry date? (reply: 2)
6. TUNEL false positive - (reply: 1)
7. So what is fluorescence SUPPOSED to look like? - fluorescent secondary antibody scans as SOLID BLACK image (reply: 1)
8. IFA of cell surface markers - IFA of osteoclasts not working, help! (reply: 2)
9. antibody against His-tagged protein - (reply: 3)
10. Mixing fixatives? - (reply: 1)
11. Antibodies, ascites and antisera - (reply: 1)
12. Casein for IF and IP - for blocking (reply: 1)
13. drying after antigen retrieval? - (reply: 3)
14. mAB vs pAB - (reply: 4)
15. Histotech "hands on" interview? - anyone given/taken a "slide test"? (reply: 4)
16. Combine 2 different primary antibodies produced by the same animal - Is it possible to succeed the impossible? (reply: 7)
17. 0.5% triton X-100 in ELISA - (reply: 1)
18. Monoclonal Ab Western and ELISA issue - (reply: 2)
19. Harvesting of DCs - (reply: 1)
20. explaination of flow cytometry histogram - urgent need (reply: 2)
21. Dendritic cell growth medium - (reply: 3)
22. monoclonal antibody (mAb), mouse, IgG2a - Urgent (reply: 3)
23. Pre-coated Poly-L-Lysine Coverslips - Does anyone know where I can get these from? (reply: 2)
24. Controls - (reply: 2)
25. coating coverslips with Poly D Lysine for ICC - How many hours/minutes would be sufficient? (reply: 1)
26. paraformaldehyde fixation - (reply: 4)
27. Cryosectioning skeletal muscle - I have huge problems with tissue damage (reply: 3)
28. anyone knows good HA antibody for zebrafish whole mount stainging - (reply: 4)
29. Using 2 polyclonals (one rabbit, one goat) in double staining? - (reply: 2)
30. Protocol for H&E staining for histopathology - (reply: 2)
2. Signal in the citoplasm instead of in the nucleus - (reply: 1)
3. Is it possible to preserve polylisine coated glasses? - (reply: 1)
4. Immunohistochemistry--How to quantify it? - (reply: 2)
5. Griess Assay reagents - Expiry date? (reply: 2)
6. TUNEL false positive - (reply: 1)
7. So what is fluorescence SUPPOSED to look like? - fluorescent secondary antibody scans as SOLID BLACK image (reply: 1)
8. IFA of cell surface markers - IFA of osteoclasts not working, help! (reply: 2)
9. antibody against His-tagged protein - (reply: 3)
10. Mixing fixatives? - (reply: 1)
11. Antibodies, ascites and antisera - (reply: 1)
12. Casein for IF and IP - for blocking (reply: 1)
13. drying after antigen retrieval? - (reply: 3)
14. mAB vs pAB - (reply: 4)
15. Histotech "hands on" interview? - anyone given/taken a "slide test"? (reply: 4)
16. Combine 2 different primary antibodies produced by the same animal - Is it possible to succeed the impossible? (reply: 7)
17. 0.5% triton X-100 in ELISA - (reply: 1)
18. Monoclonal Ab Western and ELISA issue - (reply: 2)
19. Harvesting of DCs - (reply: 1)
20. explaination of flow cytometry histogram - urgent need (reply: 2)
21. Dendritic cell growth medium - (reply: 3)
22. monoclonal antibody (mAb), mouse, IgG2a - Urgent (reply: 3)
23. Pre-coated Poly-L-Lysine Coverslips - Does anyone know where I can get these from? (reply: 2)
24. Controls - (reply: 2)
25. coating coverslips with Poly D Lysine for ICC - How many hours/minutes would be sufficient? (reply: 1)
26. paraformaldehyde fixation - (reply: 4)
27. Cryosectioning skeletal muscle - I have huge problems with tissue damage (reply: 3)
28. anyone knows good HA antibody for zebrafish whole mount stainging - (reply: 4)
29. Using 2 polyclonals (one rabbit, one goat) in double staining? - (reply: 2)
30. Protocol for H&E staining for histopathology - (reply: 2)