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Band Related Discussions
  1. the curious case of the disappearing x-ray bands - (reply: 3)
  2. A single band after sonication? - (reply: 2)
  3. band in -ve rtPCR - (reply: 2)
  4. Maxiprep troubleshooting - There are 3 bands in CsCl gradient after ultracentrifuge (reply: 1)
  5. Three RNA bands after isolation - (reply: 2)
  6. No band is visible with BamHI and XbaI Digestion - (reply: 3)
  7. RNA gel - too many bands?? (reply: 3)
  8. How to separate very close protein bands - (reply: 5)
  9. No targeted band - (reply: 1)
  10. No bands observed on anti-His western blot - (reply: 10)
  11. question about MSP bands - (reply: 7)
  12. single band -> multiple banding in PCR - (reply: 2)
  13. Optimum annealing temperature were fail with no band and just smearing - Optimum annealing temperature were fail with no band and just smearing (reply: 18)
  14. Ladder Fine but no DNA bands please help! - (reply: 6)
  15. How to get GOOD rt-pcr bands - (reply: 7)
  16. PCR Puzzle- Unusal Bands - (reply: 13)
  17. Problem in RT-PCR - getting a band in the negative control (reply: 4)
  18. protein band is shifted after boiling sample with sample buffer - (reply: 7)
  19. protein band is shifted after boiling sample with sample buffer - (reply: 4)
  20. Can we store the gel slice after cutting out the band and before gel extraction? - (reply: 3)
  21. Collagen bands in Western Blot - (reply: 2)
  22. Signals on Molecular Weight Bands? - (reply: 1)
  23. CsCl ultracentrifugation - No satellite band (reply: 2)
  24. Non specific bands on western blot. - (reply: 2)
  25. Wiggly 5 kDa band Western blot - (reply: 1)
  26. can't view the protein band..help! - (reply: 1)
  27. non-specific bands in western blotting - (reply: 3)
  28. Large bands (207kDa) showing up in CoIP western - (reply: 1)
  29. Is it possible to use a PCR cleaning kit to clean a band from a TAE-Agarose gel? - (reply: 1)
  30. very bright non-specific band - (reply: 7)
  31. no high molecular weight bands seen.. know to be present.. - where did they go.. in a gradeint gel? (reply: 3)
  32. Strange large bands on my PCR gel, has anyone seen this before? - PCR (reply: 3)
  33. Ghost bands on SDS gel - Problem with blur bands (reply: 5)
  34. emsa with ebox probe - multiple bands shifted - (reply: 4)
  35. Plasmid DNA preparation - two DNA bands in gel (reply: 5)
  36. High concentration DNA on spectrophotometer but no band on agarose gel. WHere is - DNA from bone and teeth (reply: 5)
  37. band size with flag tagged proteins? - (reply: 3)
  38. Pipette Like band SDS PAGE - (reply: 3)
  39. Mystery band after Phenol:Chloro:IAA Extraction after restriction digest - (reply: 3)
  40. Trouble with Western Blot to detect secreted enzyme - what is causing the band pattern? (reply: 2)
  41. Immunization with protein band from SDS-PAGE - (reply: 5)
  42. using piece agarose band directly on PCR - does it work? - (reply: 3)
  43. bands sizes with sectreted protein - (reply: 1)
  44. Why does insert has two bands after RE digestion? - (reply: 3)
  45. Wester blot non specific bands caused by secondary - (reply: 3)
  46. No band after Double digestion - (reply: 4)
  47. Problem with bands of PCR products - (reply: 2)
  48. no bands, no ladders, nothing at all in my gels - (reply: 7)
  49. SDS PAGE Double Bands for >6 months - (reply: 6)
  50. always two bands in colony PCR - What the hell did another band come from? (reply: 4)
  51. 2 bands in same location - (reply: 5)
  52. only one band after double enzyme digestion - (reply: 4)
  53. Post sonication RNAse & Prot K treatment - What are the bands? (reply: 15)
  54. ponceau staining bands - (reply: 1)
  55. Band Intensity - Linear or Exponential? PCR Products - (reply: 4)
  56. WB reverse image solution?! - white band on darck background (reply: 2)
  57. two bands for actin? - (reply: 3)
  58. PCR faint bands under UV light - (reply: 10)
  59. SDS Gel - My protein bands go to the bottom of gel (reply: 9)
  60. no band after digestion - (reply: 5)
  61. Dimer bands under reducing conditions - (reply: 1)
  62. PCR inespecific band amplification - (reply: 2)
  63. alpha tubulin band the wrong size! - (reply: 2)
  64. Strange run or transfer of proteins at 15% PAGE (not band but spot) - (reply: 2)
  65. RNA gel - multiple bands - (reply: 2)
  66. native gel problem: blurry bands - (reply: 11)
  67. southern blot of plant genomic dna - no band detected! percentage of homology matter? (reply: 15)
  68. IP band slightly different size than input - (reply: 4)
  69. SDS-PAGE --problems in resolving bands - (reply: 7)
  70. Protein G purified IgG>>> extra band at approx 200 kd - (reply: 5)
  71. 3 bands on WB instead of 1 - (reply: 4)
  72. Separation of DNA bands of sizes 8.86 kb and 9.5 kb - (reply: 3)
  73. disappear protein band after development - (reply: 11)
  74. sds page bands appearing below expected for crp - sds page bands appearing below expected (reply: 4)
  75. No bands in non-reducing SDS-PAGE - (reply: 9)
  76. SDS gel image : why the band looks like 'stapler bullet' - (reply: 3)
  77. Bands in Negative control PCR - (reply: 18)
  78. DNA band Breakage - (reply: 2)
  79. Only 18S RNA Band shows up in RNA gel, why? - (reply: 2)
  80. peptid conjugation to BSA ? No band in the gel? - (reply: 1)
  81. BSP to test imprinted genes DNA methylation of oocytes - no specific or smear bands! (reply: 4)
  82. more protein can give a band shift on a SDS-PAGE? - (reply: 1)
  83. How to quantify Western blot results/bands? - with ImageJ? Scion? Other programs? (reply: 4)
  84. Antibodies for different epitope giving bands of different sizes in the same sam - (reply: 1)
  85. ChIP: bright 300bp band after RNAse A - (reply: 1)
  86. uneven bands from Immunoprecipitation - (reply: 5)
  87. Co-Ip - bands in my pre-clearing controls - pre-clearing step - how to improve it? (reply: 2)
  88. plz guide me.i got many band in one well in agrose gel electrophoresis - PCR (reply: 8)
  89. Could you run Taqman PCR on gel to see the band? - (reply: 1)
  90. extra bands on SDS PAGE gel - (reply: 4)
  91. Gap between bands too small - DNA Gel Electrophoresis (reply: 6)
  92. Faint Band - PCR reaction - (reply: 2)
  93. protein band is shifted after incubation with plant protein extracts! - (reply: 2)
  94. WESTERN BLOT : upper band at 45 kDa in blot of cytochrome c - (reply: 2)
  95. Mab purification - no mouse IgG band seen (reply: 3)
  96. bands/dyes don't move on gel - (reply: 9)
  97. Problems with several unspecific bands after PCR - (reply: 2)
  98. No bands seen when probing for P-JNK - (reply: 4)
  99. no band in 6% gel - (reply: 4)
  100. EMSA problem - differently shifted bands (reply: 2)
  101. using ImageJ for densitometry - how to quantitate bands using imageJ (reply: 1)
  102. Band sizes after co-IP - (reply: 4)
  103. waves bands on the sds-page - (reply: 3)
  104. Problems with DIG DNA labeling! help! - Membrane was completely stained purple with no bands (reply: 12)
  105. Uneven band migration on agarose gel - (reply: 4)
  106. band disappearance on agarose gel - (reply: 3)
  107. Formaldehyde Gel Electrophoresis on non-denatured/denatured RNA - No/Faint RNA bands after denatration (reply: 1)
  108. EMSA probe: two bands in free probe lane - (reply: 1)
  109. RNA Denaturation and Electrophoresis - RNA Bands Disappear after denaturation!!! (reply: 4)
  110. Strange "V" band in Gel Shift - (reply: 4)
  111. getting faint band during ligation - (reply: 7)
  112. Detection of a small phospho protein - Not getting any band at all. (reply: 4)
  113. Western Blot Hollow "Ghost" Bands with ECF - (reply: 1)
  114. No bands after coomassie blue staining - (reply: 1)
  115. peptide expresion, but no western blot bands. - (reply: 2)
  116. shaded bands in agarose gel - (reply: 8)
  117. cloning faint bands - (reply: 5)
  118. Weird two bands of my purified vector DNA apeared on my gel ? - (reply: 5)
  119. Northern Blot - no specific bands - (reply: 2)
  120. Very odd western bands - (reply: 4)
  121. Multiple bands in PCR - (reply: 6)
  122. Why do I got 3 bands of RNA gel? - (reply: 3)
  123. Why 2 bands for housekeeping? - western blot (reply: 2)
  124. Positive bands in both M and U for MSP - have tried everything - please help! (reply: 9)
  125. western blot- some bands not seen - western blot (reply: 1)
  126. identifying a gel band by mass spectrometre? - problem in identifying the protein, no matches (reply: 13)
  127. How to get rid of non-specific bands on Western blots. - (reply: 9)
  128. Lack of bands in SDS-PAGE gel - (reply: 4)
  129. Multiple bands in WB - (reply: 2)
  130. band running at wrong(?) size! - more than one band, and at almost twice the predicted size. (reply: 6)
  131. Cloning/Miniprep Blues - High molecular weight bands iand no plasmid n my miniprep (reply: 3)
  132. do you subtract gel base intensity from PCR band intensity? - urgent require (reply: 3)
  133. blurry, smeared bands on my western blots - new to the world of the westerns (reply: 4)
  134. Western blot problem--- bar shape band - (reply: 5)
  135. PCR Trouble Shooting - new primer, wrong dilution of primers, wrong band, and many more (reply: 15)
  136. gst purification, got extra band - (reply: 4)
  137. Western blot: Circular bands - artefact? - (reply: 4)
  138. Acrylamide gel with abnormal band - (reply: 4)
  139. Western Blotting using Anti α Tubulin antibody: Bands too high! - (reply: 3)
  140. Northern blot band size calculation - (reply: 1)
  141. mass spec with silver stain band, unsecssessful. help!1 - (reply: 7)
  142. RT PCR produces nice crisp band but of wrong length :-) - (reply: 2)
  143. Smad4 - double band on Western blot - (reply: 3)
  144. Help needed. PCR band problem - (reply: 4)
  145. Problem with SDS-PAGE-diffuse protein bands - (reply: 4)
  146. RT-PCR: Band in no reverse transcriptase lane - (reply: 18)
  147. 'ladder bands' in PCR - (reply: 11)
  148. PAGE gel DNA band shape - (reply: 4)
  149. western on VEGF... I see no bands at all... ned help - (reply: 4)
  150. PFGE help - bands only running half way - (reply: 3)
  151. no bands in PCR reaction analysis - does centrifuge time matter? (reply: 2)
  152. EMSA results have other bands in control DNA - (reply: 1)
  153. PCR inespecific band only in control - why only in the control? (reply: 2)
  154. Trouble with ugly bands in EMSA - (reply: 1)
  155. Straight line selection - defining area for western blot bands - (reply: 3)
  156. band densities - (reply: 4)
  157. densitometry of western blot bands - (reply: 2)
  158. Bands in IEF gel - How can I extract them? (reply: 3)
  159. faint band of interest & primer-dimer prob - (reply: 5)
  160. SDS PAGE band migrates smalle when reduced?! - (reply: 2)
  161. plasmid bands - (reply: 1)
  162. Why dpes uncut (undigested) DNA show more than one band in gel-electrophoresis? - Need to know the reason why it shows multiple bands (reply: 1)
  163. Why does uncut dna show more than one band? - why does uncu dna show more than one band? (reply: 2)
  164. Strong bands at 220 bp after sonication - (reply: 3)
  165. Which bands for pET in agarosegel ? - (reply: 1)
  166. no bands in sds page - (reply: 2)
  167. Additional bands on SDS-PAGE under reducing conditions - (reply: 1)
  168. Very resistand band in PCR - (reply: 7)
  169. no DNA band - (reply: 4)
  170. No bands in RFLP - (reply: 2)
  171. scanner for quantification of western bands - (reply: 1)
  172. MSP-M bands in healthy people - (reply: 8)
  173. Actin bands not equal on Western blot - (reply: 5)
  174. PCR cloning, faint band - (reply: 4)
  175. Problem with maxiprep-no band! - (reply: 2)
  176. low MW bands left on gel during transfer to PVDF - (reply: 1)
  177. confusing shape of survivin protein band - (reply: 3)
  178. Troubleshoot: Western; no bands appear in reduced lanes - (reply: 8)
  179. quantify with imageJ - unequal size of bands (reply: 2)
  180. Strange phenomenon with western blot - Double bands (reply: 2)
  181. HELP: Excision of gel band from EMSA gel - (reply: 1)
  182. silver stain can even get band with the loading buffer control - (reply: 4)
  183. white bands in wb - (reply: 4)
  184. ha-tag purification , lots of bands - (reply: 4)
  185. blue light-sybr green - anybody see bands with sybr and blue light (reply: 3)
  186. Many negative bands in WB - (reply: 4)
  187. DNA-PAGE - U-shaped bands (reply: 4)
  188. In gel electrophoresis, what are the effects of sample loading on band size? - (reply: 3)
  189. Multiple Bands in Westren Blot? - My antibodies are polyclonal...is dat d reason im getting multiple bands? (reply: 2)
  190. Protein purification for rabbit injection - SDS-PAGE gel, excise band, inject into rabbit?? (reply: 5)
  191. ugly bands on sds-page of high mw protein (mTOR) - (reply: 9)
  192. help as to why there are no bands when running my PCR product - (reply: 3)
  193. Lots of bands on immunoblot for an expressed protein - (reply: 8)
  194. Ghost bands in Western Blots - (reply: 5)
  195. protein bands are not migrating over to the whole gels. - please suggest me something, very urgent ! (reply: 8)
  196. Quantity One for bands - (reply: 5)
  197. mobility of protein band over PAGE - please suggest (reply: 1)
  198. pcr product afetr reverse transcriptase reaction - 4 bands i have not included the band which i want??? (reply: 5)
  199. crooked bands on SDS-PAGE - (reply: 3)
  200. Analysis of DNA fingerprints-software - Image interpretation, bands size,... (reply: 2)
  201. GST purification multiple band problem - GST purification problem, multiple band (reply: 3)
  202. Problems with PCR for GATEWAY - I'd get rid of 100-150bp unspecific band!!! (reply: 5)
  203. GST-purification multiple band problem - GST-purification (reply: 1)
  204. "Phantom" GFP bands - (reply: 2)
  205. Qiagen DNA Kits - Extra bands (reply: 7)
  206. multiple non specific bands - (reply: 3)
  207. weak band during denature agarose gel electrophoresis of RNA - RNA denature agarose gel electrophoresis (reply: 3)
  208. weird DNA bands in the Minipreps; competent cells contamination? - (reply: 3)
  209. analysing gel bands - analysing gel bands (reply: 1)
  210. same vector but diffirent clones produce bands of different size. - (reply: 4)
  211. Smeary DNA band on agarose gel - (reply: 5)
  212. problems with PCR from chIP material - double band PCR from chIP material (reply: 2)
  213. Expected bands in agarose gel electrophoresis - (reply: 3)
  214. Fuzzy bands on agarose gel after ligation - (reply: 7)
  215. SDS-PAGE gel interpretation - sds page gel band reading (reply: 3)
  216. protease bands on the gel.... - should they be visible? (reply: 11)
  217. Gel and Ct-values relationship: is there a probe problem? - Bands on gel don't fit my expectations... (reply: 2)
  218. PCR band for water but no contamination, Digestion not working - (reply: 10)
  219. darker bands in WB - (reply: 6)
  220. No supershift stronger band - (reply: 10)
  221. bands bend in SDS PAGE - (reply: 18)
  222. No bands in WB - (reply: 2)
  223. Transformation of a plasmid into E.coli BL21 - problem! - Unexpected bands (reply: 7)
  224. PCR product multiple bands - (reply: 3)
  225. Purified protein (his-tagged) multiple bands on gel - all picked up by antibody - what could they be? (reply: 2)
  226. more ethidium bromide, brighter band? - (reply: 4)
  227. Absence of bands in some lanes of gel electrophoresis - What are the reasons? (reply: 2)
  228. curved bands in my WB - (reply: 8)
  229. Standard PCR banding? - multiple bands instead of one (reply: 4)
  230. 3 bands after digestion - (reply: 13)
  231. mutiple DNA bands on my agarose gel - (reply: 3)
  232. HMW band in well after bisulfite PCR - (reply: 1)
  233. Multiple bands on Western blot for an expressed protein - (reply: 7)
  234. western-blot: unspecific bands - How to reduce the amount of unspecific bands??? (reply: 6)
  235. NF-kB protein nuclear and cytosol isolation - No band separation in cytosol and nucleus (reply: 7)
  236. Double bands in PCR - WHY? - (reply: 5)
  237. sear in PCR no bands - (reply: 3)
  238. fading DNA bands on agarose gel - is it normal? - (reply: 4)
  239. Immunoprecipitation-I can't see the heavy and light chain bands - (reply: 1)
  240. Single Worm PCR - faint band after doing the technique (reply: 5)
  241. One or two bands with different protein extraction buffer - (reply: 3)
  242. help separate 2 DNA bands of only 100 bp difference and elution ! - already tried 2% but some thing wrong help!! (reply: 8)
  243. PCR band disappears in agarose gel! - Agarose gel electrophoresis (reply: 4)
  244. A question about supershift assay - Band disappeared but no supershift band (reply: 5)
  245. diffuse bands on western blots - (reply: 5)
  246. How to store PAGE gel and keep the bands after gel is run? - (reply: 22)
  247. contaminating bands in Ni affinity purification - (reply: 5)
  248. PFGE: how do i improve band clarity? - (reply: 1)
  249. PCR almost like false positive problem! - How can I get rid of 5bp band near mine !!!!!!! (reply: 2)
  250. Bands appear poor/not at all and do not appear consistantly & primer bands a - (reply: 2)
  251. Dsitinct Bands on Ponceau Stain After Sonication? - (reply: 4)
  252. NI-NTA colunm purification - no bands after purification (reply: 2)
  253. "Ponceau S" distorting the bands in western blot? - (reply: 5)
  254. SDS-PAGE - did not find the protein band what I expect (reply: 3)
  255. Why can I not see bands on an agarose gel? - agarose gel visualization (reply: 5)
  256. One specific DNA band became 2 bands after gel extraction? - (reply: 6)
  257. Unwanted band seen along with my pcr product. - regarding PCR product with unwanted band formation during electrophoresis (reply: 3)
  258. Western band distorted by 'bubbles' - (reply: 8)
  259. Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
  260. Extra band in western blot - (reply: 1)
  261. Western blot bands fade out - (reply: 9)
  262. Stuborn band after digestion of plasmid DNA - (reply: 4)
  263. how to separate immunoreactivity and intensity of the band? - (reply: 2)
  264. double digestion problem - additional band after double digestion (reply: 2)
  265. multiple bands in western blot - (reply: 5)
  266. trouble shooting Actin Bands - (reply: 4)
  267. Purification through the Ni-NTa column, multiple band, help - (reply: 8)
  268. Not getting actin bands equal on Western blot - (reply: 11)
  269. how could one avoid amplification of a very high non-specific band from PCR - (reply: 3)
  270. just a word of advice - better to lean from others mistakes. ECL and missing bands (reply: 1)
  271. fragmented DNA bands! - fragmented DNA bands!!!!! (reply: 2)
  272. smearing in bands after digestion? - Why does a smear appear after digestion? (reply: 4)
  273. Northern is smeary but you can see 28S and 18S bands - (reply: 1)
  274. SDS-PAGE band dissolution for scintillation counting - (reply: 5)
  275. No bacterial bands in uninduced - (reply: 3)
  276. eluting protein from the gel band... - (reply: 7)
  277. Real Time: One Melting Curve 2 gel bands - (reply: 6)
  278. so my protein has degraded....why am I seeing higher bands? - (reply: 3)
  279. Two bands on RT-PCR product - (reply: 8)
  280. terrible streaks on western bands - (reply: 7)
  281. Strong distortion in bands of dna gel electrophoresis - (reply: 6)
  282. Duplicate PCR: specific bands but different sizes. - (reply: 2)
  283. Unclear bands in bp marker of agarose gel - should i lower the voltage? (reply: 5)
  284. Why two bands in my Western blotting - (reply: 1)
  285. If digestion not adequate, can I recycle the bands and digest again? - (reply: 1)
  286. urea/glycerol PAGE and cutting out the band... - cant find out protocols (reply: 1)
  287. Ladder vs Agarose - The size of the band is wrong and higher (reply: 6)
  288. Primer dimers?Contaminations? - Unexpected bands! (reply: 5)
  289. special band in BL21(DE3) - protein expression (reply: 8)
  290. Band in negative PCR control - (reply: 25)
  291. Non specific bands on Western blot - (reply: 1)
  292. Multiple bands on RNA gel, why? - (reply: 6)
  293. unexpected bands on MSP - (reply: 2)
  294. Faint band of genomic DNA in 1.5% gel - the possible cause of faint DNA band (reply: 9)
  295. Why is my smaller band too faint? Genomic PCR not working! - Just trying to genotype some mice... (reply: 2)
  296. multiple bands on WB - b-actin blots show multiple bands!!! (reply: 1)
  297. SDS-PAGE ghost band help - (reply: 1)
  298. Unspecific band in Western Blot - (reply: 11)
  299. protein band - (reply: 6)
  300. Western Blotting band - (reply: 3)
  301. RNA isolation from castor leaf sample using GTC method - unable to detect RNA bands in castor leaf sample using GTC method (reply: 1)
  302. two bands of one gene in PCR - (reply: 8)
  303. Ghost band in SDS-PAGE - Help me!!!! (reply: 21)
  304. strange band - (reply: 2)
  305. Missing rRNA bands - (reply: 3)
  306. Western blot bands look like "M" - (reply: 6)
  307. bigger band after reduction with dithithreitol - (reply: 1)
  308. western blot - missing expected band - (reply: 1)
  309. Multiple unspecifc bands in IP using Protein A sepharose + silver staining - (reply: 2)
  310. Western blot bands look like 'upside down smiles' - (reply: 4)
  311. Problem whith b-actin bands - (reply: 1)
  312. [urgent help] Band missing in PCR - (reply: 5)
  313. single clone isolate showed multiple bands in DGGE - (reply: 1)
  314. no band after long-distance PCR - (reply: 3)
  315. High MW bands are ugly - electrophoresis (reply: 5)
  316. many bands other than my protein on western... - please help me to explain this... (reply: 5)
  317. two bands after gel elusion instead of one - (reply: 6)
  318. Can Southern blotting resolve 13.7kb and 11.5kb bands? - Help! (reply: 2)
  319. antiFLAG detect non specific band not FLAG - (reply: 9)
  320. No band on double digestion of construct - (reply: 1)
  321. Two bands with MSP - (reply: 6)
  322. unknown PCR bands - (reply: 4)
  323. smear band in southern hybridization - (reply: 2)
  324. Smear after PCR - No band but smear were found from my samples (reply: 3)
  325. Smeary bands in real-time RT-PCR - (reply: 7)
  326. Gel elution of a band - (reply: 4)
  327. my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
  328. strange bands on western blot - (reply: 2)
  329. Qiagen Rneasy multiple bands in gel - (reply: 1)
  330. Band diffusion of my PCR product in electrophoresis - (reply: 3)
  331. extra band after gel extraction - (reply: 4)
  332. Strange PCR bands behaviour - (reply: 2)
  333. RNA Isolation using Trizol - Getting an additional band and v.low 260/230 (reply: 2)
  334. Plasmid appears as two bands after double digestion - (reply: 2)
  335. Western problem - very challenging - distorted band, non-reproducible, generally awful (reply: 5)
  336. white band on WB - Western blot (reply: 6)
  337. multiplex pcr trouble - some bands won't amplify (reply: 16)
  338. Miniprep plasmid shows three bands - (reply: 1)
  339. Vertical bands - (reply: 3)
  340. Weird Band gel migration - Molecular biology (reply: 7)
  341. NATIVE gel band - sample running at the side... - My NATIVE gel run produced a band which has hollowed out central with visible st (reply: 1)
  342. faint pcr bands to some, intense to some - (reply: 5)
  343. Wrong band size using precast gel - (reply: 4)
  344. No bands at all in gel for PCR products - (reply: 5)
  345. lingo question regarding "artifact" bands on western - what does it all mean? (reply: 3)
  346. Narrow bands while running SDS-PAGE - (reply: 1)
  347. Western Blot troubleshooting: band is too high - LiCor imaging of Alpha tubulin membranes (reply: 6)
  348. Foreign band after double-digestion - (reply: 4)
  349. Im stuck with my PCR for nearly 2 months! - i've no bands!! (reply: 5)
  350. Weak Western band for phosphorylated Akt - whats happening?? (reply: 17)
  351. white bands on western blots - (reply: 1)
  352. Detection of approx. 300 kDa band on SDS-PAGE - MLL (reply: 3)
  353. Two bands on a denaturing gel - always two bands on a denaturing gel with double stranded substrate? (reply: 1)
  354. RNA Gel Problems - RNA not showing up as bands in gel (reply: 6)
  355. mysterious band after transformation and miniprep... - (reply: 11)
  356. Urea-PAGE for transcription assays - Diffusion of low molecular weight bands (reply: 2)
  357. Cloning PCR product - double bands or heterozygote (reply: 5)
  358. no band in PCR ! - (reply: 4)
  359. do you always see three bands on your miniprep? - (reply: 3)
  360. Mystery band after vector digestion - (reply: 3)
  361. duplet bands in western blot - advice (reply: 5)
  362. Double-digesting my vector - should I see a 76bp band? (reply: 6)
  363. Dimers and weird bands after Bis-PCR - (reply: 5)
  364. Lower size band in genomic PCR with 300 bp missing - (reply: 12)
  365. Some lanes see bands,other no, strange! - western blotting questions (reply: 10)
  366. Western band on blot uncapturable by film - (reply: 3)
  367. Genomic DNA on agarose gel - two bands of genomic DNA on agarose gel (reply: 2)
  368. About MSP result ! - why always have two bands (reply: 3)
  369. non-specific bands in agarose gel - too many bands visible! (reply: 3)
  370. Very dense primer-dimer band but no product - (reply: 4)
  371. Primer extension problem - there are too many bands!! (reply: 1)
  372. Western blot bands look like dumbells - (reply: 2)
  373. shift of protein bands in SDS gel electrophoresis - where does it come from, can it be improved (reply: 2)
  374. faint bands of PCR products - (reply: 5)
  375. nonspecific band after purification - (reply: 2)
  376. how can I take this non specific band out?? - lysis did not go well (reply: 6)
  377. no band obtain after PCR - (reply: 2)
  378. multiple band on PCR - (reply: 2)
  379. Larger than expected bands on a Western - What could they be? (reply: 2)
  380. Can I separate these 2 large DNA bands? - (reply: 1)
  381. Weak competition in EMSA - 330 times molar excess, but still strong bands (reply: 7)
  382. RNA gel - 28S band (reply: 2)
  383. No band on BSP - (reply: 17)
  384. Compare DNA bands - (reply: 1)
  385. WB: one single band but at the wrong size... - what to make of it? (reply: 4)
  386. quantify bands - (reply: 4)
  387. Problems in CHIP assay? - How can i get rid of multiple bands in CHIP assay? (reply: 3)
  388. Do you think I can separate these 2 DNA bands? - mol. biol. (reply: 13)
  389. aspecific bands in cellculture protein, not in tissue - sds page (reply: 3)
  390. AFLPs - few bands in AFLPs (reply: 2)
  391. SDS-PAGE did not show bands. High ion strength? - (reply: 3)
  392. My antibody recognizes two bands - I tried everything and I have still two bands (reply: 2)
  393. EMSA gel - dirty bands! (reply: 7)
  394. wrong sized bands/smear in both nested MSP PCRs - (reply: 5)
  395. Too many bands on Western blot - (reply: 5)
  396. Distorted bands on Western Blot - (reply: 10)
  397. Too many unspecific bands with B-actin - Western Blot (reply: 5)
  398. How to get sharp band on agarose gel? - (reply: 7)
  399. lot of contaminating bands, what should i do - (reply: 2)
  400. express a big protein in BL21, get an extra band - (reply: 7)
  401. Problems with MSP - Unable to pick up bands for methylation (reply: 1)
  402. Sometimes i get bands, sometimes i get nothing for my PCR - Strange PCR Results (reply: 1)
  403. Monoclonal antibody but getting multiple bands??? - (reply: 3)
  404. relative DNA quantification from PCR - calculation of band intensity from PCR (reply: 2)
  405. mutilple bands after RT-PCR - (reply: 2)
  406. mysterious 1.6kb band after transformation - (reply: 1)
  407. Unspecific bands after gel purification, why? - (reply: 7)
  408. DNA binding by RE causes bigger band than substrate in gel? - Restriction digest of 500bp frag. with BsaXI (reply: 4)
  409. quantitation of PCR bands - (reply: 1)
  410. 1% formaldehyde gel bands - (reply: 1)
  411. His-Tag purification using Qiagen Ni-NTA spin column - Extra band of unknown protein beside target protei (reply: 2)
  412. Southern problem - No signal - Where are my bands?----southern blotting (reply: 6)
  413. 3 bands on RT-PCR - (reply: 4)
  414. ABC technique with Western blot - Do you get extra band with ABC? (reply: 1)
  415. SDS-PAGE of protein from membrane fraction - problems with protein band profile (reply: 1)
  416. band spread in western - (reply: 9)
  417. small sized restricted bands couldnt be visualized - restriction enzymes problems (reply: 22)
  418. the protein band disappear in the second dimension - 2D-Gel (reply: 2)
  419. Shadow bands? - (reply: 6)
  420. Extrremely bright bands in the WELLs of PCR agarose gel - Bright and sharp bands in the PCR gel well (reply: 9)
  421. PCR problem: DISTINCT bands far shorter than target! - (reply: 3)
  422. RNAse protection assay - No bands (reply: 2)
  423. Phospho-Elk1 specific Antibody...which band?? - (reply: 2)
  424. non-specific band in ChIP assay - (reply: 1)
  425. PCR and electrophoresis - help finding missing bands! (reply: 3)
  426. bands from tissue one size, from cells - size doubles - help! (reply: 4)
  427. How to determine band intensity of Western blot? - High School Student in need of help for the Science Fair! (reply: 8)
  428. Bands on Western are not sharply defined - (reply: 6)
  429. DIG Northern - faint bands and high background (reply: 2)
  430. AFLP double bands in bad profiles - (reply: 2)
  431. digest problem - extra bands (reply: 2)
  432. DGGE and Smiling bands! - Side bands on my DGGE gel keep smiling! (reply: 2)
  433. please tell me how to eliminate interfering band ? - (reply: 1)
  434. Electrophoresis - negative bands - (reply: 1)
  435. PCR - No bands anymore in well functioning PCR - (reply: 6)
  436. The weirdest PCR ever! - 8kb band in 1min. (reply: 3)
  437. Negative bands - (reply: 3)
  438. After restriction digestion my bands just disappear - (reply: 3)
  439. Erroneous agarose gel bands - (reply: 2)
  440. TA cloning -- funny sized bands - (reply: 1)
  441. EMSA/ band shift enquiries - EMSA/ band shift enquiries (reply: 1)
  442. Problems with TOPO cloning - I don't get the right band size. (reply: 4)
  443. no template but still got exact size bands???!!! - PCR genotyping problem (reply: 1)
  444. multiple bands from monoclonal antibody - (reply: 1)
  445. my bands spread as a line trough the gel - (reply: 3)
  446. cloning size is not correct - 2 bands on the PCR product (reply: 2)
  447. Bands are like a "w" - (reply: 1)
  448. too many bands in western blotting - (reply: 2)
  449. Band in the negative control - (reply: 1)
  450. Western Blotting Problem - High band (reply: 1)
  451. smears instead of bands - (reply: 2)
  452. correct bands in RT-PCR negative control - (reply: 14)
  453. Double Bands in Western Blotting - Please Help (reply: 2)
  454. PCR problem - can not get the target SSR band (reply: 1)
  455. Quantification of density of DNA bands - (reply: 3)
  456. aspecific band of BSA in western blot - western blotting (reply: 1)
  457. Nested PCR - 2 bands present (reply: 2)
  458. Ethidium bromide DNA gel staining - rapidly fading bands (reply: 1)
  459. No 28s rRNA band in agarose gel? - (reply: 1)
  460. Band Shift Optimisation - (reply: 2)
  461. Three bands after Ni agarosecolumn - (reply: 1)
  462. plasmid mini-prep from E.coli - smeared bands (reply: 2)
  463. Re: Elution retarded band - (reply: 7)
  464. Pcr cloning - how to get a single band (reply: 4)
  465. trouble in plasmid pcr - i cannot obtain designed band from a definied plasmid (reply: 4)
  466. A single band is observed afterthe isolation of RNA by kit...! - (reply: 1)
  467. band shift assay dig labelledDNA - (reply: 1)
  468. phantom band in DNA purification - A new band appears after purifying DNA from agarose gels (reply: 1)
  469. Band shift assay - (reply: 1)