Protocol Online logo
Top : Forum Archives: : Indexed by Topic
ELISA Related Discussions
  1. how do u determine actual concentration from absorbance readings? - using tissue lysates for ELISA sample (reply: 2)
  2. ELISA protocol (indirect and sandwich) and troubleshooting tips - from abcam (reply: 3)
  3. ELISA - how to make a protein lysate? - (reply: 3)
  4. Steroid coating - I'm looking a protocol for steroid ELISA. (reply: 3)
  5. choosing the best ELISA method - used to do Immunoprecipitation (reply: 2)
  6. ELISA standards. - (reply: 2)
  7. ELISA result interpretation - (reply: 2)
  8. eNOS ELISA - (reply: 2)
  9. ELISA Results - (reply: 1)
  10. Cell ELISA, Permeabilization cell - (reply: 1)
  11. ELISA protocol for testing HIV/AIDS - Anyone know how I can get qualified to do this? (reply: 2)
  12. Can a 96 well plate for rt PCR be used for ELISA? - 96 well plate , ELISA (reply: 2)
  13. help ELISA! - color all over plate - (reply: 3)
  14. hGH ELISA - (reply: 1)
  15. Using H2O to wash ELISA plate? - (reply: 7)
  16. Lysis Buffer for Elisa - (reply: 1)
  17. MIP-2 ELISA detailed questions - (reply: 3)
  18. ELISA Problem since change of antibody supplier? - (reply: 1)
  19. Monoclonal antibody affinity (Kd constant) determination using ELISA - (reply: 2)
  20. Elisa for total human IgG from culture supernatant - (reply: 1)
  21. elisa background - (reply: 3)
  22. ELISA vs Western - The two aren't agreeing.. (reply: 1)
  23. Sandwich ELISA - IgY quantification - (reply: 3)
  24. Please help me with ELISA - Need help with ELISA basics and instrumentation (reply: 4)
  25. Dot-blot ELISA - (reply: 1)
  26. Coating ELISA Plate.. Tips please - (reply: 3)
  27. Help! Question regarding last step of ELISA - (reply: 3)
  28. ELISA, high background from serum with no Ag - Help needed please!! (reply: 4)
  29. ELISA sensitivity - how to increase sensitivity of ELISA (reply: 1)
  30. Indirect ELISA false positives? - (reply: 3)
  31. ELISA protocol - (reply: 4)
  32. Need help with ELISA! I am a newbie! - (reply: 5)
  33. Western vs. ELISA - (reply: 4)
  34. PGE2 ELISA - (reply: 1)
  35. Optical density variation in indirect ELISA - (reply: 3)
  36. Do I need to do BCA assay to quantify the protein before doing the ELISA? - BCA assay and ELISA (reply: 9)
  37. RIPA as Extraction Buffer in ELISA - (reply: 3)
  38. Concentrating dilute protein samples for use in elisa - (reply: 2)
  39. how to quantify total protein in Elisa? - Elisa (reply: 1)
  40. Influence bacteria on ELISA - (reply: 1)
  41. ELISA optimization problems - ELISA (reply: 3)
  42. Career with ELISA - (reply: 8)
  43. ECL reagents in ELISA - (reply: 6)
  44. Corticosterone antigen as standard for Direct ELISA - (reply: 4)
  45. comparison of ELISA protocols - (reply: 6)
  46. Indirect IgM ELISA Background - (reply: 1)
  47. How to get rid of bubbles on ELISA plates? - (reply: 9)
  48. ELISA ALTERNATIVE - (reply: 9)
  49. ELISA background problem - (reply: 11)
  50. ELISA and phage display - (reply: 7)
  51. Why capture ELISA failed - (reply: 5)
  52. Interpretation of ELISA Data\Producing a Standard Curve - (reply: 7)
  53. WB vs ELISA ? - (reply: 2)
  54. ScFv ELISA data not right - (reply: 2)
  55. Blank controls in ELISA? - (reply: 5)
  56. i need a nice protocol for celluler ELISA - (reply: 3)
  57. ELISA standard curve - approximate protein concs to use? - (reply: 3)
  58. discordance between western blot and ELISA - (reply: 10)
  59. Counting cells using ELISA method? - Counting cells using ELISA method (reply: 1)
  60. Signal disappearance in ELISA - (reply: 8)
  61. Human interferon-beta ELISA - (reply: 1)
  62. Conditioned media, filter or centrifuge? - which way is the best way to prep for ELISA (reply: 4)
  63. how long is too long for ELISA develping? - (reply: 2)
  64. how long is too long for ELISA develping? - (reply: 3)
  65. ELISA problems - No attachment of antigen (reply: 2)
  66. Specific wavelenght to find optical density - Specific Wavelenght to determine optical density in ELISA (reply: 4)
  67. Semen testing in ELISA - (reply: 4)
  68. ELISA in a real-time PCR plate - possible? (reply: 3)
  69. ELISA/ BSA in capture antibody storage buffer - (reply: 3)
  70. protein concentration for ELISA - (reply: 1)
  71. denatured antigen for elisa - (reply: 3)
  72. ELISA inhibition/competition assay - (reply: 1)
  73. ELISA antibody-use for WB? - (reply: 6)
  74. ELISA Standard curve question - (reply: 3)
  75. recovery rate of ELISA - (reply: 1)
  76. cellular ELISA - cellular ELISA involving determing Ab concentration (reply: 1)
  77. elisa coating antigen preparing - (reply: 1)
  78. ELISA diluent options - (reply: 4)
  79. an ELISA procedure for Measuring murine serum Ab titer - does anybody know of such an ELISA protocol or kit? (reply: 2)
  80. Urgent - IFN gamma by ELISA (reply: 1)
  81. Cellular ELISA - (reply: 4)
  82. Four-parameter logistic software - I need the software to generate ELISA standard curve... (reply: 2)
  83. Background due to biotin label in ELISA - (reply: 3)
  84. ELISA Testosterone - How to determine hormone level? (reply: 11)
  85. ELISA - new kit without coating buffer - (reply: 3)
  86. ELISA Data Analysis Question - (reply: 1)
  87. ELISA Develop. Problems with Capture Sandwich Technique - (reply: 1)
  88. ELISA/Dot blot for detection of Methylated DNA - (reply: 3)
  89. Trouble with Elisa calibration curve - ??? (reply: 6)
  90. IFNgamma ELISA standard curve - (reply: 9)
  91. Non-specific binding in a Sandwich ELISA - (reply: 3)
  92. ELISA with serum - (reply: 3)
  93. ELISA Question - Finding the concentration of Ab from cell culture (reply: 4)
  94. Serum-plasma dilution in ELISA sandwich - (reply: 1)
  95. What is the sensitivity of dot blot compared to ELISA, and what antibody concent - (reply: 1)
  96. Serum ELISA IgG titer quantitation - How to determine IgG titer without known sample? (reply: 4)
  97. post-immunization ELISA standards question - (reply: 3)
  98. alpha-fetoprotein ELISA problem - (reply: 1)
  99. ELISA and Falcon tubes - (reply: 3)
  100. ELISA protocol - (reply: 2)
  101. How do I prepare a sample to quantify a certain protein with ELISA? - (reply: 10)
  102. western vs elisa - (reply: 4)
  103. peptide quantification in plasma by ELISA - (reply: 3)
  104. ELISA Ab question - denatured protein and extract - If I extract denatured protein... (reply: 2)
  105. ELISA with virus as antigen - (reply: 2)
  106. Competetive ELISA - (reply: 4)
  107. ELISA problems - sample recovery! - why is this happening? (reply: 3)
  108. ELISA Incubation time? - (reply: 2)
  109. rat Cytokine ELISA - (reply: 1)
  110. ELISA coating question - (reply: 4)
  111. Elisa wavelength - (reply: 2)
  112. sensitivity and specificity of ELISA - (reply: 2)
  113. High background in sandwich ELISA? - (reply: 3)
  114. Sandwich ELISA antibodies - (reply: 3)
  115. Can antibody recommended for ELISA or WB be used on IHC? - (reply: 5)
  116. Low OD's for ELISA - (reply: 2)
  117. ELISA IL12 p70 from mouse dendritic cells culture SN - (reply: 2)
  118. Two different antibodies necessary for sandwich ELISA? - (reply: 3)
  119. Biogenic amine ELISA - Non-peptide ELISA (reply: 2)
  120. ELISA EGFR ligands (EGF, amphiregulin and TGF-alpha) - Advice on setup ELISA EGFR ligands (reply: 1)
  121. ELISA background - (reply: 2)
  122. problem with elisa - (reply: 2)
  123. Human Active MMP-8 ELISA - (reply: 1)
  124. Immunofluorescence microscopy, Western Blot, ELISA, ... - (reply: 4)
  125. ELISA and RNA purification - Can ELISA be performed on frozen leafs (reply: 4)
  126. How to detect the antigenicity of peptide? - except ELISA (reply: 7)
  127. ELISA and BSA - Non-ELISA grade BSA (reply: 15)
  128. how to reduce the background in the ELISA assay? - using peptide as antigen (reply: 18)
  129. Magnetic particle ELISA - few questions - Seems to work well, but not being used much?? (reply: 3)
  130. Antibody Position on ELISA plate - (reply: 7)
  131. ELISA wash buffer alternates - (reply: 2)
  132. bovine serum sample in ELISA - (reply: 2)
  133. lysate for ELISA can be used for WESTERN? - (reply: 2)
  134. ELISA with fixed bacterial cells - (reply: 1)
  135. Human albumin ELISA - all positive results (most of the time !) - (reply: 5)
  136. ELISA background - (reply: 2)
  137. history of ELISA - FROM WHERE THE NAME ELISA CAME (reply: 7)
  138. Best Plate Brand for Fluorescent Capture ELISA - (reply: 1)
  139. extract peptides from tissue/cell culture for ELISA kits - (reply: 3)
  140. ELISA standard curves and computer programs - (reply: 4)
  141. Capture ELISA - human Serum - (reply: 5)
  142. capture elisa - (reply: 4)
  143. Serum or Plasma, which is better to detect cytokines? - additionally, how about the comparation of Luminex and ELISA? (reply: 3)
  144. Basic ELISA questions - (reply: 1)
  145. streptavidin coating for ELISA - (reply: 2)
  146. ELISA on cow fecal and milk specimens - (reply: 3)
  147. Kinds of ELISA - Kinds of ELISA (reply: 3)
  148. chemistry in ELISA - (reply: 1)
  149. Basic Question regarding ELISA - (reply: 2)
  150. crp elisa - (reply: 2)
  151. ELISA background - ELISA background (reply: 3)
  152. Who have used beads covered with protein in ELISA? - Are they heavy enought to be washed out? (reply: 2)
  153. ELISA experiment - High background (reply: 1)
  154. ELISA methods - needing information/papers (reply: 4)
  155. ELISA versus ELISpot - (reply: 2)
  156. ELISA troubling shooting - (reply: 2)
  157. ELISA negativ control? - (reply: 2)
  158. Low ELISA titers - (reply: 1)
  159. Old telomerase - PCR elisa kit - can it work? - (reply: 2)
  160. very higher background in ELISA which was going on very well before - (reply: 10)
  161. VEGF ELISA - (reply: 1)
  162. ELISA for casein - need help setup ELISA for casein (reply: 8)
  163. ELISA for serum titer - Why preimmune serum has relative high background (reply: 2)
  164. ELISA with denaturated proteins? - (reply: 6)
  165. identical results in ELISA assay - I must be doing somthing wrong, but what? (reply: 18)
  166. Optimizing ELISA! - Papers and help on Optimizing my ELISA!! (reply: 1)
  167. ELISA beginner - bacterial protein (reply: 2)
  168. ELISA Plates - comparison of OD (reply: 3)
  169. Looking for ELISA kit for proteinase 3 - Not anti-proteinase 3 antibody ELISA kit (reply: 1)
  170. Interpreting ELISA results - (reply: 2)
  171. Statistics for interpreting ELISA results - Statistics for interpreting ELISA results (reply: 2)
  172. opinion on CBA kits - good replacement for ELISA? (reply: 2)
  173. Can bacteria lysate be used to test a serum ab titer by ELISA? - Need suggestion (reply: 3)
  174. Thoughts on this experimental design - CAT elisa (reply: 1)
  175. ELISA reading range - ELISA assay (reply: 1)
  176. Help on ELISA antibody binding principles - (reply: 2)
  177. ELISA weak reaction - ELISA problem (reply: 1)
  178. No endpoint in Indirect Peptide ELISA! - (reply: 1)
  179. EDTA/Heparin as antocoagulant for plasam ELISA - EDTA/Heparin as antocoagulant for plasam ELISA (reply: 1)
  180. About Elisa - (reply: 1)
  181. question about cellular Elisa - (reply: 2)
  182. ELISA controls - (reply: 1)
  183. anti-dsDNA ELISA - (reply: 1)
  184. Polyclonal vs. Monoclonal antibody for Sandwich ELISA? - (reply: 10)
  185. Main forces linkage in ELISA methods - about Abs/Ags with polystyren (reply: 3)
  186. Immunofluorescent ELISA - (reply: 2)
  187. Non-specific binding in ELISA assays - Reducing background with human serum (reply: 24)
  188. Elisa - (reply: 1)
  189. Rat TNFa ELISA problems - (reply: 1)
  190. TMB in ELISA at 630nm - (reply: 1)
  191. ELISA - direct protein assay with ELISA (reply: 1)
  192. Cell Lysate ELISA - Find protocol for a cell-lysate ELISA (reply: 2)
  193. IS  there any protocol forantigen detection by compititive ELISA - (reply: 2)
  194. GFP ELISA essay - (reply: 1)
  195. ELISA incubator - need an incubator or oven for capturing antibody (reply: 1)
  196. Elisa - (reply: 1)
  197. sandwich elisa - (reply: 1)
  198. ELISA problems - Coating and OD (reply: 1)
  199. Protocol of ELISA - (reply: 1)
  200. ELISA for frozen fat tissue - (reply: 1)