|
1. Validation of MicroRNA array results - (reply: 1)
2. qRT-PCR for pre- and pri-miRNAs - (reply: 2)
3. Exiqon miRNA arrays- any experiences - difficulties in validation (reply: 4)
4. Transfected copies per cell - (reply: 1)
5. mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
6. naked shRNA transfection - (reply: 2)
7. Double SiRNA trasfection - (reply: 1)
8. Troubles in transfection methods! - All cells die before making a selection (reply: 5)
9. can shRNA expression set share one polyA terminator with other gene - (reply: 3)
10. mirvana isolation kit - (reply: 5)
11. confusion about off-target effect - (reply: 3)
12. Why do miRNAs always bind to 3'UTR? - (reply: 3)
13. Sirna concentration - (reply: 1)
14. suggested siRNA concentrations - (reply: 1)
15. RNAi of non-coding RNAs....? - (reply: 6)
16. Invitrogen's Negative control siRNA - (reply: 3)
17. Establishing siRNA Experiments - controls, sequences...recommendations (reply: 3)
18. Ethanol or isoprapanol to precipitate miRNA? - (reply: 1)
19. Gene Knockout - (reply: 2)
20. Dissolve siRNA in non-RNase free buffer - (reply: 1)
21. Spacing of artificial miRNA target sites for efficient knockdown - (reply: 1)
22. miRNA 3' end nucleotidylation - (reply: 4)
23. CD4 siRNA - (reply: 1)
24. Inducible Lentiviral systems - (reply: 1)
25. gene dowregulation by miRNA - (reply: 1)
26. SMP30 silencing in vivo - stem cell biology (reply: 2)
27. How to construct miRNA expression vector - (reply: 1)
28. Discovery of novel miRNAs - (reply: 1)
29. miRNA profiling - (reply: 6)
30. Optimization of siRNA experiments - (reply: 1)
2. qRT-PCR for pre- and pri-miRNAs - (reply: 2)
3. Exiqon miRNA arrays- any experiences - difficulties in validation (reply: 4)
4. Transfected copies per cell - (reply: 1)
5. mirVana™ qRT-PCR miRNA Detection versus miScript SYBR Green PCR kit - advantages and disadvantages (reply: 1)
6. naked shRNA transfection - (reply: 2)
7. Double SiRNA trasfection - (reply: 1)
8. Troubles in transfection methods! - All cells die before making a selection (reply: 5)
9. can shRNA expression set share one polyA terminator with other gene - (reply: 3)
10. mirvana isolation kit - (reply: 5)
11. confusion about off-target effect - (reply: 3)
12. Why do miRNAs always bind to 3'UTR? - (reply: 3)
13. Sirna concentration - (reply: 1)
14. suggested siRNA concentrations - (reply: 1)
15. RNAi of non-coding RNAs....? - (reply: 6)
16. Invitrogen's Negative control siRNA - (reply: 3)
17. Establishing siRNA Experiments - controls, sequences...recommendations (reply: 3)
18. Ethanol or isoprapanol to precipitate miRNA? - (reply: 1)
19. Gene Knockout - (reply: 2)
20. Dissolve siRNA in non-RNase free buffer - (reply: 1)
21. Spacing of artificial miRNA target sites for efficient knockdown - (reply: 1)
22. miRNA 3' end nucleotidylation - (reply: 4)
23. CD4 siRNA - (reply: 1)
24. Inducible Lentiviral systems - (reply: 1)
25. gene dowregulation by miRNA - (reply: 1)
26. SMP30 silencing in vivo - stem cell biology (reply: 2)
27. How to construct miRNA expression vector - (reply: 1)
28. Discovery of novel miRNAs - (reply: 1)
29. miRNA profiling - (reply: 6)
30. Optimization of siRNA experiments - (reply: 1)