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Digestion Related Discussions
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restriction enzyme digestion - (reply: 5)
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Digestion, clean up lead to fewer DNA! Help... - (reply: 4)
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dam methylation? - digestion problem (reply: 3)
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double digestion time - (reply: 1)
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Restriction Enzyme digestion - (reply: 4)
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failed restriction digests - (reply: 11)
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killer digest issues - (reply: 8)
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Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
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Digestion of antibody using pepsin - no F(ab)2 was found (reply: 1)
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Digest with BglII and SalI - (reply: 1)
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plasmid digestion gel - (reply: 4)
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Genome Restriction Digestion - (reply: 2)
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Digestion problems with BamH1 - .... in a linearised vector.... (reply: 3)
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M13 Hind and Sal digest - (reply: 2)
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Digesting ethanol precipitated DNA with AseI - (reply: 2)
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double digestion and ligation - (reply: 3)
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Multimerization after restriction digestion - (reply: 9)
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Help, sequestial digest - (reply: 1)
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HELP WITH RESTRICTION DIGEST OF PHAGE DNA - (reply: 4)
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Double digest and 3' end polishing with klenow - (reply: 1)
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how to do serial digestion with EcoR1 and bamH1 - (reply: 5)
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No band after Double digestion - (reply: 4)
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Miniprep DNA digestion smear - (reply: 15)
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all the DNA gone after digestion - (reply: 8)
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Vector digestion - (reply: 6)
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no band after digestion - (reply: 5)
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problems with restriction digestion - (reply: 7)
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Ecor1/Pst1 digest - (reply: 4)
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Problems with double digestion - (reply: 1)
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can hyaluronidase be used to detach/digest cells? - (reply: 1)
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DNA smears after digestion - DNA smears after digestion (reply: 6)
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P/C/I extraction of plasmid after digestion - (reply: 1)
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4 enzyme digest and 3 piece ligation? - (reply: 3)
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Problem in Restriction Digestion... - why is it cutting something it is not supposed to?? (reply: 1)
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Cloning without any digestion and ligation prosedures - GATEWAY Cloning Technology (reply: 3)
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cloning a 3kb fragment into a 6kb vector - or maybe an EcoRI/HindIII double digest problem? (reply: 3)
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DNA degradation while digestion. - (reply: 3)
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Proteases and thier site cleavage - Enzyme digestion (reply: 2)
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Integrity of overhang after digestion - (reply: 3)
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How to digest my cell line/strain to a single-cell suspension? - Help! (reply: 4)
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Is this clone the correct one? - Restriction digestion doubt (reply: 1)
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NotI / EcoRI digest - (reply: 5)
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pET28b digestion problem - pET28b plasmid with CASC2a sequence (reply: 2)
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lyzozyme digest - HPLC - HPLC digest Lyzozyme (reply: 1)
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Nde1 works on positive control, not on DNA sample - Restriction Digest (reply: 6)
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Double digestion - (reply: 13)
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unable to digest my recombinant plasmid! - (reply: 9)
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plasmid DNA Digestion - (reply: 5)
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Zymography/Detecting pro-Zymogen - How does pro-enzyme digest the substrate? (reply: 2)
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restriction digestion - (reply: 5)
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Restriction enzyme digestion problem - (reply: 4)
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partial digestion of Tth111I - difficult??? (reply: 2)
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Does my protein degrade during DNAse I and thrombin digestion? - (reply: 1)
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Problem with Digestion or Ligation. How to tell? - (reply: 7)
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Digestion in sub-optimal buffer - (reply: 5)
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Double RE digest: using two different companies - (reply: 3)
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Restriction enzyme digestion - procedures in restriction enzyme digestion (reply: 6)
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Protecting against Bgl II digestion - (reply: 2)
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Sequential digestion - (reply: 3)
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Can restriction digestion be replaced by CHIP? - (reply: 5)
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Digestion, Ligation or transformation problem? - (reply: 8)
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Nla III digestion - (reply: 2)
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Base pairs apart between 2 sites for sucessful double digestion? - (reply: 2)
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digesting DNA with buffer incompatible enzymes - (reply: 4)
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How to check double digest efficiency of a linear PCR product? - Molecular Biology (reply: 8)
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I need help with the steps in sequential digests please - (reply: 3)
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How do you work out a restriction digestion map for a circular DNA plasmid? - (reply: 2)
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Is a Triple Restriction Digest Possible? - (reply: 6)
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AscI digestion of plasmid - Problem with AscI digestion of plasmid DNA (reply: 6)
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ppt DNA directly from a digestion/ pcr - (reply: 2)
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RE digest and klenow - sequential blunting (reply: 6)
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which restriction enzyme should I use to digest the vector? - (reply: 3)
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forgot to digest PCR insert before ligation. - what happened? (reply: 7)
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Is my restriction digest not cutting properly? - (reply: 8)
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PCR band for water but no contamination, Digestion not working - (reply: 10)
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After double digest my plasmid is 2Kbp shorter! - (reply: 3)
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My single restriction enzyme digestion doesn't work - Digestion problem, no cutting. (reply: 32)
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3 bands after digestion - (reply: 13)
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Restriction digestion by SpeI and SphI - (reply: 2)
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Few colonies with cloning of PCR product after digestion - (reply: 7)
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Religation with single digest vector - (reply: 7)
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problems digesting genomic DNA from cell lines - (reply: 1)
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PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
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Tryptic digest help - Trouble diffusing stain (reply: 3)
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Problem with DNase digestion of RNA - (reply: 6)
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Digestion or Ligation problems, Help! - Help!!!!!! (reply: 5)
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BAC digestion - (reply: 8)
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Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
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disappearing vector after digestion - (reply: 11)
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Stuborn band after digestion of plasmid DNA - (reply: 4)
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single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
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How far 2 restriction sites should be? - how many bases in between for efficient digestion. (reply: 4)
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Full and Partial Digestion - (reply: 3)
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Basic Question Restriction Digest - (reply: 11)
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Digestion Problem with BsmBI - (reply: 3)
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If digestion not adequate, can I recycle the bands and digest again? - (reply: 1)
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Digesting PCR products - (reply: 2)
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KpnI and BamHI double digest help - (reply: 6)
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stability of protein sample after in-gel digestion - (reply: 2)
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How to choose HindIII/EcoRI double digest buffer? - a stupid question, but I really curious about the answer (reply: 6)
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keep getting incomplete digest for plasmid - (reply: 3)
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CHIP questions - proteinase K digestion and DNA shearing - (reply: 2)
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Effect of bile concentration on human digestion - Human digestion (reply: 5)
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Reagents used...in restriction digestion and electrophoresis - (reply: 2)
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restriction digestion of genomic poplar dna - digestion problems with genomic dna of poplar (reply: 3)
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RT-PCR, DNA contaminant removal by digestion with restriction enzyme - (reply: 3)
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Problems with Southern hybridization and restriction digest of genomic DNA - (reply: 2)
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Help on digestion and ligation...colonies not wanted why? - (reply: 4)
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How to extract a 20kb vector after double digestion - (reply: 4)
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Restriction digest - (reply: 6)
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No band on double digestion of construct - (reply: 1)
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BclI digestion - (reply: 4)
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basic RE digests acting weird - (reply: 3)
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Help me--why my plasmid DNA is lost after digestion with Enzyme - (reply: 1)
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RE Double Digest XhoI/NdeI TA Vector - (reply: 2)
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Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
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Cloning - RE digestion clean up (reply: 2)
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Incomplete genomic digestion for Southern - (reply: 1)
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ligation transformation of puc18 and phage lambda using ecor1 and bamh1 - general double/single digest problem (reply: 3)
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CIP with restriction digestion - (reply: 5)
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HindIII digestion - (reply: 1)
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how do you inactivate the enzymes after digestion? - (reply: 17)
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HELP! selective scission - thrombin digestion (reply: 1)
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what is your reaction system of digesting DNA? - (reply: 8)
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why we use BVS in DNA digestion? - (reply: 3)
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restriction digestion problem... - (reply: 12)
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Problem with BsaB1 digestion - (reply: 1)
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Southern - Southern Blot Digestion (reply: 4)
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what's wrong with my digestion - (reply: 2)
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digestion with two REs requiring different buffer - (reply: 10)
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Problem with restriction digestion - (reply: 10)
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How to increase the efficacy of ligation when using single RE digestion - (reply: 17)
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T4 blunting follow by digestion - (reply: 2)
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digestion with pvu1 enzyme - (reply: 3)
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Obtain larger dna fragments after digestion and gel cleaning - (reply: 2)
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Double digestion/ligation problem - (reply: 4)
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Can I directly digest PCR products? - (reply: 9)
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Question about NcoI digestion... - (reply: 3)
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Double digestion of PCR product and ligation - (reply: 5)
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Dnase I digestion - (reply: 3)
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Double digestion problem - (reply: 1)
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Use of restriction enzyme digestion before bisulfite conversion - What is the purpose of r-enzyme digestion? (reply: 2)
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problem with generation of restriction digestion site - (reply: 4)
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maximum [EDTA] in restriction digests - (reply: 2)
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Double Digestion - double digestion for southern (reply: 1)
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in- gel trypsin digestion - (reply: 1)
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digestion problems - (reply: 1)
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double digestion problem - (reply: 9)
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Help!What's wrong with the endonuclease digestion of total DNA of - (reply: 2)
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Help! E.coli GM48: digestion/DNA degradation - (reply: 2)
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Digest pUAST vector with two restriction site too closely located - too closed sites for double digest (reply: 7)
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Genomic DNA Complete Digestion - (reply: 4)
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Problems digesting with BclI and SspI - Any help is apriciated (reply: 1)
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DNase digestion and RNA degradation - Cancer tissue RNA isolation (reply: 1)
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partial digestion - match partial digestion conditions (reply: 1)
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BamH1 and Nde1 double digestion problem - (reply: 3)
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restriction digestion - (reply: 2)
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Double Digest problems - (reply: 2)
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digestion & purification problem - (reply: 6)
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genomic DNA digest - (reply: 3)
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Double digestion and ligation problem - (reply: 15)
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Restriction digestion problem!! - XbaI digestion (reply: 1)
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DNA digestion time? - how long how many units (reply: 1)
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enzymatic digestion - (reply: 3)
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Difficult double digestion - (reply: 3)
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