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Digestion Related Discussions
  1. restriction enzyme digestion - (reply: 5)
  2. Digestion, clean up lead to fewer DNA! Help... - (reply: 4)
  3. dam methylation? - digestion problem (reply: 3)
  4. double digestion time - (reply: 1)
  5. Restriction Enzyme digestion - (reply: 4)
  6. failed restriction digests - (reply: 11)
  7. killer digest issues - (reply: 8)
  8. Digestion and ligation - Do I omit the dephosphorylation steps? (reply: 4)
  9. Digestion of antibody using pepsin - no F(ab)2 was found (reply: 1)
  10. Digest with BglII and SalI - (reply: 1)
  11. plasmid digestion gel - (reply: 4)
  12. Genome Restriction Digestion - (reply: 2)
  13. Digestion problems with BamH1 - .... in a linearised vector.... (reply: 3)
  14. M13 Hind and Sal digest - (reply: 2)
  15. Digesting ethanol precipitated DNA with AseI - (reply: 2)
  16. double digestion and ligation - (reply: 3)
  17. Multimerization after restriction digestion - (reply: 9)
  18. Help, sequestial digest - (reply: 1)
  19. HELP WITH RESTRICTION DIGEST OF PHAGE DNA - (reply: 4)
  20. Double digest and 3' end polishing with klenow - (reply: 1)
  21. how to do serial digestion with EcoR1 and bamH1 - (reply: 5)
  22. No band after Double digestion - (reply: 4)
  23. Miniprep DNA digestion smear - (reply: 15)
  24. all the DNA gone after digestion - (reply: 8)
  25. Vector digestion - (reply: 6)
  26. no band after digestion - (reply: 5)
  27. problems with restriction digestion - (reply: 7)
  28. Ecor1/Pst1 digest - (reply: 4)
  29. Problems with double digestion - (reply: 1)
  30. can hyaluronidase be used to detach/digest cells? - (reply: 1)
  31. DNA smears after digestion - DNA smears after digestion (reply: 6)
  32. P/C/I extraction of plasmid after digestion - (reply: 1)
  33. 4 enzyme digest and 3 piece ligation? - (reply: 3)
  34. Problem in Restriction Digestion... - why is it cutting something it is not supposed to?? (reply: 1)
  35. Cloning without any digestion and ligation prosedures - GATEWAY Cloning Technology (reply: 3)
  36. cloning a 3kb fragment into a 6kb vector - or maybe an EcoRI/HindIII double digest problem? (reply: 3)
  37. DNA degradation while digestion. - (reply: 3)
  38. Proteases and thier site cleavage - Enzyme digestion (reply: 2)
  39. Integrity of overhang after digestion - (reply: 3)
  40. How to digest my cell line/strain to a single-cell suspension? - Help! (reply: 4)
  41. Is this clone the correct one? - Restriction digestion doubt (reply: 1)
  42. NotI / EcoRI digest - (reply: 5)
  43. pET28b digestion problem - pET28b plasmid with CASC2a sequence (reply: 2)
  44. lyzozyme digest - HPLC - HPLC digest Lyzozyme (reply: 1)
  45. Nde1 works on positive control, not on DNA sample - Restriction Digest (reply: 6)
  46. Double digestion - (reply: 13)
  47. unable to digest my recombinant plasmid! - (reply: 9)
  48. plasmid DNA Digestion - (reply: 5)
  49. Zymography/Detecting pro-Zymogen - How does pro-enzyme digest the substrate? (reply: 2)
  50. restriction digestion - (reply: 5)
  51. Restriction enzyme digestion problem - (reply: 4)
  52. partial digestion of Tth111I - difficult??? (reply: 2)
  53. Does my protein degrade during DNAse I and thrombin digestion? - (reply: 1)
  54. Problem with Digestion or Ligation. How to tell? - (reply: 7)
  55. Digestion in sub-optimal buffer - (reply: 5)
  56. Double RE digest: using two different companies - (reply: 3)
  57. Restriction enzyme digestion - procedures in restriction enzyme digestion (reply: 6)
  58. Protecting against Bgl II digestion - (reply: 2)
  59. Sequential digestion - (reply: 3)
  60. Can restriction digestion be replaced by CHIP? - (reply: 5)
  61. Digestion, Ligation or transformation problem? - (reply: 8)
  62. Nla III digestion - (reply: 2)
  63. Base pairs apart between 2 sites for sucessful double digestion? - (reply: 2)
  64. digesting DNA with buffer incompatible enzymes - (reply: 4)
  65. How to check double digest efficiency of a linear PCR product? - Molecular Biology (reply: 8)
  66. I need help with the steps in sequential digests please - (reply: 3)
  67. How do you work out a restriction digestion map for a circular DNA plasmid? - (reply: 2)
  68. Is a Triple Restriction Digest Possible? - (reply: 6)
  69. AscI digestion of plasmid - Problem with AscI digestion of plasmid DNA (reply: 6)
  70. ppt DNA directly from a digestion/ pcr - (reply: 2)
  71. RE digest and klenow - sequential blunting (reply: 6)
  72. which restriction enzyme should I use to digest the vector? - (reply: 3)
  73. forgot to digest PCR insert before ligation. - what happened? (reply: 7)
  74. Is my restriction digest not cutting properly? - (reply: 8)
  75. PCR band for water but no contamination, Digestion not working - (reply: 10)
  76. After double digest my plasmid is 2Kbp shorter! - (reply: 3)
  77. My single restriction enzyme digestion doesn't work - Digestion problem, no cutting. (reply: 32)
  78. 3 bands after digestion - (reply: 13)
  79. Restriction digestion by SpeI and SphI - (reply: 2)
  80. Few colonies with cloning of PCR product after digestion - (reply: 7)
  81. Religation with single digest vector - (reply: 7)
  82. problems digesting genomic DNA from cell lines - (reply: 1)
  83. PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
  84. Tryptic digest help - Trouble diffusing stain (reply: 3)
  85. Problem with DNase digestion of RNA - (reply: 6)
  86. Digestion or Ligation problems, Help! - Help!!!!!! (reply: 5)
  87. BAC digestion - (reply: 8)
  88. Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
  89. disappearing vector after digestion - (reply: 11)
  90. Stuborn band after digestion of plasmid DNA - (reply: 4)
  91. single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
  92. How far 2 restriction sites should be? - how many bases in between for efficient digestion. (reply: 4)
  93. Full and Partial Digestion - (reply: 3)
  94. Basic Question Restriction Digest - (reply: 11)
  95. Digestion Problem with BsmBI - (reply: 3)
  96. If digestion not adequate, can I recycle the bands and digest again? - (reply: 1)
  97. Digesting PCR products - (reply: 2)
  98. KpnI and BamHI double digest help - (reply: 6)
  99. stability of protein sample after in-gel digestion - (reply: 2)
  100. How to choose HindIII/EcoRI double digest buffer? - a stupid question, but I really curious about the answer (reply: 6)
  101. keep getting incomplete digest for plasmid - (reply: 3)
  102. CHIP questions - proteinase K digestion and DNA shearing - (reply: 2)
  103. Effect of bile concentration on human digestion - Human digestion (reply: 5)
  104. Reagents used...in restriction digestion and electrophoresis - (reply: 2)
  105. restriction digestion of genomic poplar dna - digestion problems with genomic dna of poplar (reply: 3)
  106. RT-PCR, DNA contaminant removal by digestion with restriction enzyme - (reply: 3)
  107. Problems with Southern hybridization and restriction digest of genomic DNA - (reply: 2)
  108. Help on digestion and ligation...colonies not wanted why? - (reply: 4)
  109. How to extract a 20kb vector after double digestion - (reply: 4)
  110. Restriction digest - (reply: 6)
  111. No band on double digestion of construct - (reply: 1)
  112. BclI digestion - (reply: 4)
  113. basic RE digests acting weird - (reply: 3)
  114. Help me--why my plasmid DNA is lost after digestion with Enzyme - (reply: 1)
  115. RE Double Digest XhoI/NdeI TA Vector - (reply: 2)
  116. Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
  117. Cloning - RE digestion clean up (reply: 2)
  118. Incomplete genomic digestion for Southern - (reply: 1)
  119. ligation transformation of puc18 and phage lambda using ecor1 and bamh1 - general double/single digest problem (reply: 3)
  120. CIP with restriction digestion - (reply: 5)
  121. HindIII digestion - (reply: 1)
  122. how do you inactivate the enzymes after digestion? - (reply: 17)
  123. HELP! selective scission - thrombin digestion (reply: 1)
  124. what is your reaction system of digesting DNA? - (reply: 8)
  125. why we use BVS in DNA digestion? - (reply: 3)
  126. restriction digestion problem... - (reply: 12)
  127. Problem with BsaB1 digestion - (reply: 1)
  128. Southern - Southern Blot Digestion (reply: 4)
  129. what's wrong with my digestion - (reply: 2)
  130. digestion with two REs requiring different buffer - (reply: 10)
  131. Problem with restriction digestion - (reply: 10)
  132. How to increase the efficacy of ligation when using single RE digestion - (reply: 17)
  133. T4 blunting follow by digestion - (reply: 2)
  134. digestion with pvu1 enzyme - (reply: 3)
  135. Obtain larger dna fragments after digestion and gel cleaning - (reply: 2)
  136. Double digestion/ligation problem - (reply: 4)
  137. Can I directly digest PCR products? - (reply: 9)
  138. Question about NcoI digestion... - (reply: 3)
  139. Double digestion of PCR product and ligation - (reply: 5)
  140. Dnase I digestion - (reply: 3)
  141. Double digestion problem - (reply: 1)
  142. Use of restriction enzyme digestion before bisulfite conversion - What is the purpose of r-enzyme digestion? (reply: 2)
  143. problem with generation of restriction digestion site - (reply: 4)
  144. maximum [EDTA] in restriction digests - (reply: 2)
  145. Double Digestion - double digestion for southern (reply: 1)
  146. in- gel trypsin digestion - (reply: 1)
  147. digestion problems - (reply: 1)
  148. double digestion problem - (reply: 9)
  149. Help!What's wrong with the endonuclease digestion of total DNA of - (reply: 2)
  150. Help! E.coli GM48: digestion/DNA degradation - (reply: 2)
  151. Digest pUAST vector with two restriction site too closely located - too closed sites for double digest (reply: 7)
  152. Genomic DNA Complete Digestion - (reply: 4)
  153. Problems digesting with BclI and SspI - Any help is apriciated (reply: 1)
  154. DNase digestion and RNA degradation - Cancer tissue RNA isolation (reply: 1)
  155. partial digestion - match partial digestion conditions (reply: 1)
  156. BamH1 and Nde1 double digestion problem - (reply: 3)
  157. restriction digestion - (reply: 2)
  158. Double Digest problems - (reply: 2)
  159. digestion & purification problem - (reply: 6)
  160. genomic DNA digest - (reply: 3)
  161. Double digestion and ligation problem - (reply: 15)
  162. Restriction digestion problem!! - XbaI digestion (reply: 1)
  163. DNA digestion time? - how long how many units (reply: 1)
  164. enzymatic digestion - (reply: 3)
  165. Difficult double digestion - (reply: 3)