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Digestion Related Discussions
  1. Digesting ethanol precipitated DNA with AseI - (reply: 1)
  2. digestion of pcr - (reply: 2)
  3. double digestion and ligation - (reply: 3)
  4. Thrombin digestion for GST - ppt problem (reply: 3)
  5. Multimerization after restriction digestion - (reply: 9)
  6. restriction enzyme digestion problem - (reply: 8)
  7. Help, sequestial digest - (reply: 1)
  8. help. Is this double digest right? - (reply: 5)
  9. HELP WITH RESTRICTION DIGEST OF PHAGE DNA - (reply: 4)
  10. Can digest HESCs (H1) with trypsin/EDTA? - (reply: 1)
  11. Double digest and 3' end polishing with klenow - (reply: 1)
  12. Mystery band after Phenol:Chloro:IAA Extraction after restriction digest - (reply: 3)
  13. how to do serial digestion with EcoR1 and bamH1 - (reply: 5)
  14. Why does insert has two bands after RE digestion? - (reply: 3)
  15. No band after Double digestion - (reply: 4)
  16. Miniprep DNA digestion smear - (reply: 15)
  17. only one band after double enzyme digestion - (reply: 4)
  18. all the DNA gone after digestion - (reply: 8)
  19. EcoR1-Pst1 digest for vector and insert - (reply: 3)
  20. Vector digestion - (reply: 6)
  21. how to do double digestion with EcoRI and BamHI - (reply: 9)
  22. no band after digestion - (reply: 5)
  23. BamH1-Pst1 digest - (reply: 5)
  24. problems with restriction digestion - (reply: 7)
  25. Restriciton digest protocol for adjacent sites? - (reply: 7)
  26. Ecor1/Pst1 digest - (reply: 4)
  27. For double sequential digest: which enzyme first? - (reply: 7)
  28. Problems with double digestion - (reply: 1)
  29. Help: How to isolate plasmid from agrobaterium for restriction enzyme digestion - (reply: 10)
  30. can hyaluronidase be used to detach/digest cells? - (reply: 1)
  31. Dnase digestion after in vitro transcription - (reply: 4)
  32. DNA smears after digestion - DNA smears after digestion (reply: 6)
  33. RE digestion Help needed everyone - (reply: 6)
  34. P/C/I extraction of plasmid after digestion - (reply: 1)
  35. double digestion of transformed bacteria colony - (reply: 7)
  36. 4 enzyme digest and 3 piece ligation? - (reply: 3)
  37. Does trypsin digest integral plasma membrane proteins? - (reply: 2)
  38. Problem in Restriction Digestion... - why is it cutting something it is not supposed to?? (reply: 1)
  39. how to check if restriction digestion has happened - (reply: 7)
  40. Cloning without any digestion and ligation prosedures - GATEWAY Cloning Technology (reply: 3)
  41. Digestion dilemma - (reply: 5)
  42. cloning a 3kb fragment into a 6kb vector - or maybe an EcoRI/HindIII double digest problem? (reply: 3)
  43. Restriction digestion and DNA degradation problem - Restriction digestion and DNA degradation problem (reply: 1)
  44. DNA degradation while digestion. - (reply: 3)
  45. HELP in Restriction Enzyme Digestion - troubleshooting re digestion (reply: 3)
  46. Proteases and thier site cleavage - Enzyme digestion (reply: 2)
  47. Digestion question - (reply: 1)
  48. Integrity of overhang after digestion - (reply: 3)
  49. PstI/SapI digestion problem - restriction enzyme (reply: 3)
  50. How to digest my cell line/strain to a single-cell suspension? - Help! (reply: 4)
  51. Double restriction enzyme digest - (reply: 3)
  52. Is this clone the correct one? - Restriction digestion doubt (reply: 1)
  53. SacII digestion - (reply: 1)
  54. NotI / EcoRI digest - (reply: 5)
  55. DNA loss in digestion - I lose lot of DNA in digestion (reply: 10)
  56. pET28b digestion problem - pET28b plasmid with CASC2a sequence (reply: 2)
  57. Problems with restriction enzyme digest. - (reply: 2)
  58. lyzozyme digest - HPLC - HPLC digest Lyzozyme (reply: 1)
  59. Partial Digestion and Religation - Partial Digestion to eliminate a restriction site (reply: 1)
  60. Nde1 works on positive control, not on DNA sample - Restriction Digest (reply: 6)
  61. DNase Digestion and Reverse Transcription - (reply: 10)
  62. Double digestion - (reply: 13)
  63. double digestion - (reply: 6)
  64. unable to digest my recombinant plasmid! - (reply: 9)
  65. Double Digestion with Single Restriction Enzyme - (reply: 2)
  66. plasmid DNA Digestion - (reply: 5)
  67. NsiI digestion problems - NsiI is not working properly? (reply: 1)
  68. Zymography/Detecting pro-Zymogen - How does pro-enzyme digest the substrate? (reply: 2)
  69. problem with restriction digestion - (reply: 3)
  70. restriction digestion - (reply: 5)
  71. PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
  72. Restriction enzyme digestion problem - (reply: 4)
  73. Concerning digestion enzymes - How do digestion enzymes work really? (reply: 1)
  74. partial digestion of Tth111I - difficult??? (reply: 2)
  75. partial digestion of Tth111I - (reply: 1)
  76. Does my protein degrade during DNAse I and thrombin digestion? - (reply: 1)
  77. digestion and ligation with BspH1 - digestion with BspH1 of insert , and Nco1 for the vector ,ligation pos (reply: 2)
  78. Problem with Digestion or Ligation. How to tell? - (reply: 7)
  79. urgent HincII digestion problem - (reply: 5)
  80. Digestion in sub-optimal buffer - (reply: 5)
  81. Looking for a protocol for a partial digest w/ NdeI - (reply: 3)
  82. Double RE digest: using two different companies - (reply: 3)
  83. Double digest question for ligation - (reply: 4)
  84. Restriction enzyme digestion - procedures in restriction enzyme digestion (reply: 6)
  85. Spec-ed/Loaded DNA inconsistency after digestion of mini-preps - (reply: 2)
  86. Protecting against Bgl II digestion - (reply: 2)
  87. Restriction digestion - (reply: 2)
  88. Sequential digestion - (reply: 3)
  89. What is wrong with my EcoRI digestion? - Can it attribute to the star activity? (reply: 6)
  90. Can restriction digestion be replaced by CHIP? - (reply: 5)
  91. Restriction digest of pQE plasmid - Double digestion problem (reply: 7)
  92. Digestion, Ligation or transformation problem? - (reply: 8)
  93. double digestion of plasmid - problem with incomplete digestion (reply: 9)
  94. Nla III digestion - (reply: 2)
  95. DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
  96. Base pairs apart between 2 sites for sucessful double digestion? - (reply: 2)
  97. Help! my "plasmid" won't cut. - RE digestion problem (reply: 7)
  98. digesting DNA with buffer incompatible enzymes - (reply: 4)
  99. double digestion with BamHI and HindIII - (reply: 13)
  100. How to check double digest efficiency of a linear PCR product? - Molecular Biology (reply: 8)
  101. RNAse and DNA digestion in Southern Blot - (reply: 3)
  102. I need help with the steps in sequential digests please - (reply: 3)
  103. Multiple ligation - How many digestion products is it possible to ligate at the same time ? (reply: 6)
  104. How do you work out a restriction digestion map for a circular DNA plasmid? - (reply: 2)
  105. Can any one help me with restriction digestion map for DNA? - (reply: 3)
  106. Is a Triple Restriction Digest Possible? - (reply: 6)
  107. Restriction digestion of PCR product - (reply: 3)
  108. AscI digestion of plasmid - Problem with AscI digestion of plasmid DNA (reply: 6)
  109. Protocol for DNA Methylation analysis using restriction enzyme digest - (reply: 10)
  110. ppt DNA directly from a digestion/ pcr - (reply: 2)
  111. Restriction digest - volume of restriction digest (reply: 2)
  112. RE digest and klenow - sequential blunting (reply: 6)
  113. Help with AVRII digestion - (reply: 1)
  114. which restriction enzyme should I use to digest the vector? - (reply: 3)
  115. digesting pQE30 vector gives inexplicable results - (reply: 3)
  116. forgot to digest PCR insert before ligation. - what happened? (reply: 7)
  117. Methylated DNA Interfering with Restriction Digestion - Restriction digestion problems (reply: 3)
  118. Is my restriction digest not cutting properly? - (reply: 8)
  119. can I take some of ligation mix and digest? - (reply: 4)
  120. PCR band for water but no contamination, Digestion not working - (reply: 10)
  121. digest, blunt end, religate, digest.... - so many steps, maybe can skip purification... (reply: 2)
  122. After double digest my plasmid is 2Kbp shorter! - (reply: 3)
  123. Restriction digestion - (reply: 7)
  124. My single restriction enzyme digestion doesn't work - Digestion problem, no cutting. (reply: 32)
  125. Transforming after digesting PCR product - (reply: 6)
  126. 3 bands after digestion - (reply: 13)
  127. Hepg2 cells resistant to trypsin digestion - (reply: 7)
  128. Restriction digestion by SpeI and SphI - (reply: 2)
  129. PacI digestion - (reply: 3)
  130. Few colonies with cloning of PCR product after digestion - (reply: 7)
  131. digesting out restriction site - (reply: 6)
  132. Religation with single digest vector - (reply: 7)
  133. Identify insert by restriction digestion - (reply: 6)
  134. problems digesting genomic DNA from cell lines - (reply: 1)
  135. Problems with SalI digestion - (reply: 5)
  136. PCR shows insert, but digestion of plasmid failed to cut out insert - (reply: 2)
  137. Do I have to purify vector after RE digestion for cloning? - (reply: 17)
  138. Tryptic digest help - Trouble diffusing stain (reply: 3)
  139. Subcloning problem with XhoI digested fragment - weird pattern of migration/digestion after miniprep (reply: 6)
  140. Problem with DNase digestion of RNA - (reply: 6)
  141. RE digest and subsequent ligation - A base is now missing from restriction site - how, why? (reply: 7)
  142. Digestion or Ligation problems, Help! - Help!!!!!! (reply: 5)
  143. trouble with restriction digestion - (reply: 3)
  144. BAC digestion - (reply: 8)
  145. Restriction digest as a template - (reply: 2)
  146. Wrong sized PCR band following digest & ligation into vector - :( please help... (reply: 1)
  147. Problem with BamHI/SalI double digestion - (reply: 8)
  148. disappearing vector after digestion - (reply: 11)
  149. restriction digestion of PCR product? - (reply: 10)
  150. Stuborn band after digestion of plasmid DNA - (reply: 4)
  151. double digestion problem - additional band after double digestion (reply: 2)
  152. single restriction enzyme digestion? - is it possible to insert PCR product? (reply: 17)
  153. NdeI double digestion - (reply: 3)
  154. How far 2 restriction sites should be? - how many bases in between for efficient digestion. (reply: 4)
  155. Double digest: efficiency - what does 50% active in given buffer mean? (reply: 1)
  156. Full and Partial Digestion - (reply: 3)
  157. smearing in bands after digestion? - Why does a smear appear after digestion? (reply: 4)
  158. Basic Question Restriction Digest - (reply: 11)
  159. ApaI / Not I double digestion problem - (reply: 5)
  160. Digestion Problem with BsmBI - (reply: 3)
  161. Vortexing restriction digests? - (reply: 5)
  162. If digestion not adequate, can I recycle the bands and digest again? - (reply: 1)
  163. Cloning a PCR product after digestion - (reply: 7)
  164. Digesting PCR products - (reply: 2)
  165. dimers of dna (not primers) following digest or pcr? - (reply: 11)
  166. KpnI and BamHI double digest help - (reply: 6)
  167. Pronase digestion - (reply: 1)
  168. stability of protein sample after in-gel digestion - (reply: 2)
  169. Would ExoI work? - Alternative nuclease for DNA digestion (reply: 1)
  170. How to choose HindIII/EcoRI double digest buffer? - a stupid question, but I really curious about the answer (reply: 6)
  171. problems in a double digestion with NheI and EcoRI - Cloning problem (reply: 13)
  172. keep getting incomplete digest for plasmid - (reply: 3)
  173. double digest pcDNA(+) by XhoI&XbaI, uncompletely? - (reply: 3)
  174. CHIP questions - proteinase K digestion and DNA shearing - (reply: 2)
  175. How to fill in 5` overhangs after Restriction digestion - (reply: 2)
  176. Effect of bile concentration on human digestion - Human digestion (reply: 5)
  177. restriction digestion problems - digestion problems with SalI (reply: 2)
  178. Reagents used...in restriction digestion and electrophoresis - (reply: 2)
  179. protein digestion - (reply: 1)
  180. restriction digestion of genomic poplar dna - digestion problems with genomic dna of poplar (reply: 3)
  181. trouble with methlase sensitve restriction digests on plasmids isolated from DAM - (reply: 2)
  182. RT-PCR, DNA contaminant removal by digestion with restriction enzyme - (reply: 3)
  183. do you sonicate or digest with lysozyme? - which one do you prefer and why.... (reply: 21)
  184. Problems with Southern hybridization and restriction digest of genomic DNA - (reply: 2)
  185. Lipase enzyme digestion of milk experiment - effects of stale milk - Help needed understanding how stale milk could affect my experiment... (reply: 2)
  186. Help on digestion and ligation...colonies not wanted why? - (reply: 4)
  187. problem with digestion and ligation of BgI II and kpnI RE - (reply: 1)
  188. How to extract a 20kb vector after double digestion - (reply: 4)
  189. double digestion and dephosphorylation - (reply: 5)
  190. Restriction digest - (reply: 6)
  191. Interpreting RE digest results after blunt cloning - (reply: 1)
  192. No band on double digestion of construct - (reply: 1)
  193. does glycogen inhibit enzyme digestion or T4 ligation? - (reply: 11)
  194. BclI digestion - (reply: 4)
  195. Digestion Problem - Cloning (reply: 5)
  196. basic RE digests acting weird - (reply: 3)
  197. Nhe1 Restriction digestion - (reply: 3)
  198. Help me--why my plasmid DNA is lost after digestion with Enzyme - (reply: 1)
  199. tricky digestion for cloning gurus - (reply: 13)
  200. RE Double Digest XhoI/NdeI TA Vector - (reply: 2)
  201. partial digestion problem - (reply: 8)
  202. Digestion and ligation problem - Insertion of 4Kb DNA fragment into a 10Kb vector (reply: 2)
  203. my insert is there by PCR check but no band after digestion... - vector not digested because of contamination? (reply: 3)
  204. Cloning - RE digestion clean up (reply: 2)
  205. do primers and dNTPs alter digestion efficiency? - (reply: 4)
  206. Incomplete genomic digestion for Southern - (reply: 1)
  207. Plasmid appears as two bands after double digestion - (reply: 2)
  208. ligation transformation of puc18 and phage lambda using ecor1 and bamh1 - general double/single digest problem (reply: 3)
  209. Partial digest to destroy an enzyme site - (reply: 10)
  210. CIP with restriction digestion - (reply: 5)
  211. to purify these PCR products or not? - preparation for restriction digest (reply: 5)
  212. HindIII digestion - (reply: 1)
  213. Bisulfite conversion - necessary to digest DNA? - (reply: 1)
  214. how do you inactivate the enzymes after digestion? - (reply: 17)
  215. Double Digestion and Single Digestion same result? - Confusion about Plasmid and Insert alignment (reply: 4)
  216. HELP! selective scission - thrombin digestion (reply: 1)
  217. gel purification after restriction digestion - (reply: 3)
  218. what is your reaction system of digesting DNA? - (reply: 8)
  219. Digesting PCR products and cleaning up digestions - a few quick questions (reply: 4)
  220. why we use BVS in DNA digestion? - (reply: 3)
  221. Retroviral Vector won't digest ideas! - (reply: 1)
  222. restriction digestion problem... - (reply: 12)
  223. unpredictable plasmid - can digest but not sequence. . . (reply: 4)
  224. Problem with BsaB1 digestion - (reply: 1)
  225. how to denature protein without SDS and precipitating - PNGase A digestion help!!! urgent! (reply: 1)
  226. Southern - Southern Blot Digestion (reply: 2)
  227. restriction digest and DNA degradation - (reply: 3)
  228. what's wrong with my digestion - (reply: 2)
  229. Mystery band after vector digestion - (reply: 3)
  230. digestion with two REs requiring different buffer - (reply: 10)
  231. Sequential digestion - Sequential digestion using two RE with different incubation temperatures and dif (reply: 11)
  232. Problem with restriction digestion - (reply: 10)
  233. problems with digesting insert from vector - (reply: 4)
  234. How to increase the efficacy of ligation when using single RE digestion - (reply: 17)
  235. restriction digestion - (reply: 2)
  236. T4 blunting follow by digestion - (reply: 2)
  237. Question on digestion - (reply: 4)
  238. digestion with pvu1 enzyme - (reply: 3)
  239. genomic DNA PCR - RE digestion of gDNA before amplification (reply: 3)
  240. Obtain larger dna fragments after digestion and gel cleaning - (reply: 2)
  241. double digestion - Time for digestion (reply: 1)
  242. Double digestion/ligation problem - (reply: 4)
  243. restriction enzyme digestion problem - (reply: 7)
  244. Can I directly digest PCR products? - (reply: 9)
  245. Restriction Digest Woes - (reply: 1)
  246. Question about NcoI digestion... - (reply: 3)
  247. double digest not working? - BamHI+SacI double digest (reply: 4)
  248. Double digestion of PCR product and ligation - (reply: 5)
  249. what's wrong? Plasmid digestion produce only smear - (reply: 6)
  250. Dnase I digestion - (reply: 3)
  251. Plasmid digestion - Problem with digestion (reply: 3)
  252. Double digestion problem - (reply: 1)
  253. troubleshooting Rsa-I restriction digests - (reply: 2)
  254. Use of restriction enzyme digestion before bisulfite conversion - What is the purpose of r-enzyme digestion? (reply: 2)
  255. digestion problem - incomplete digestion of transformed plasmid (reply: 1)
  256. problem with generation of restriction digestion site - (reply: 4)
  257. Digest, refill an digestion - How can I do it? (reply: 2)
  258. maximum [EDTA] in restriction digests - (reply: 2)
  259. tRNA and digestion - (reply: 1)
  260. Double Digestion - double digestion for southern (reply: 1)
  261. double digestion - double digestion (reply: 2)
  262. in- gel trypsin digestion - (reply: 1)
  263. DNA binding by RE causes bigger band than substrate in gel? - Restriction digest of 500bp frag. with BsaXI (reply: 4)
  264. digestion problems - (reply: 1)
  265. Dodgy Digest - (reply: 3)
  266. double digestion problem - (reply: 9)
  267. Sequential digestion with NheI and XhoI - Restrictive Digestion (reply: 3)
  268. Help!What's wrong with the endonuclease digestion of total DNA of - (reply: 2)
  269. problems purifying plasmid DNA after restriction digestion - (reply: 3)
  270. Help! E.coli GM48: digestion/DNA degradation - (reply: 2)
  271. DNA precipitated out in digest reaction - (reply: 1)
  272. Digest pUAST vector with two restriction site too closely located - too closed sites for double digest (reply: 7)
  273. BamH1 and Xho1 double digestion problem - digestion problem (reply: 7)
  274. Genomic DNA Complete Digestion - (reply: 4)
  275. digest problem - extra bands (reply: 2)
  276. Problems digesting with BclI and SspI - Any help is apriciated (reply: 1)
  277. Enzyme digestion before bisulfite treatment - (reply: 1)
  278. DNase digestion and RNA degradation - Cancer tissue RNA isolation (reply: 1)
  279. Double digest with EcoR1 - would it work? - (reply: 1)
  280. partial digestion - match partial digestion conditions (reply: 1)
  281. Online tool for restriction digestion of DNA - (reply: 5)
  282. BamH1 and Nde1 double digestion problem - (reply: 3)
  283. After restriction digestion my bands just disappear - (reply: 3)
  284. restriction digestion - (reply: 2)
  285. A Digestion problem - (reply: 6)
  286. Double Digest problems - (reply: 2)
  287. Digestion time and enzyme - (reply: 2)
  288. digestion & purification problem - (reply: 6)
  289. Proteinase digestion of tissue for DNA extraction - (reply: 3)
  290. genomic DNA digest - (reply: 3)
  291. Methylation and restriction digestion problem - (reply: 8)
  292. Double digestion and ligation problem - (reply: 15)
  293. Phages - How to digest lambda DNA (reply: 1)
  294. Restriction digestion problem!! - XbaI digestion (reply: 1)
  295. Restriction digest - (reply: 1)
  296. DNA digestion time? - how long how many units (reply: 1)
  297. Digestion of Plasmid - Xho-I digestion of Ligated product (reply: 1)
  298. enzymatic digestion - (reply: 3)
  299. Cant digest my vector - Enzyme digest failing (reply: 5)
  300. Difficult double digestion - (reply: 3)
  301. The detailed digestion site map of pCO1 or pHaMDR1/A - (reply: 1)