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1. a gene instead an other.. - (reply: 1)
2. Got problem with maxiprep - (reply: 3)
3. selective preciptation of DNA according to size - (reply: 4)
4. dam methylation? - digestion problem (reply: 3)
5. how long does it take a colony inoculation to reach log phase? - (reply: 2)
6. Calculating transformation efficiency? - (reply: 1)
7. Calculating copy number? - (reply: 1)
8. Cloning into pGBKT7 vector - (reply: 4)
9. southern blotting problem with DIG - southern blotting problem with DIG (reply: 4)
10. pRARE2 vector sequence - (reply: 4)
11. Inverse PCR ligation - (reply: 1)
12. SapI enzyme - (reply: 3)
13. gene expresion - (reply: 1)
14. can you transform directly into liquid culture? - (reply: 2)
15. Is Pet22b from Novagen low copy? - (reply: 1)
16. ligation problem by using single RE - (reply: 8)
17. Plasmid DNA concentration - (reply: 2)
18. blunt-end ligation - (reply: 2)
19. False positives in clonation: long strain insert inside my colonies, please help - (reply: 1)
20. klenow/t4 and dNTPs - question (reply: 1)
21. Size of expressed Protein - (reply: 2)
22. DNA precipitation EtOH with Na OAc or Ammonium OAc - (reply: 4)
23. Phosphatasing to prevent vector reclosures - (reply: 1)
24. Troubleshooting! Strange growth in LB medium - (reply: 3)
25. Making blunt ends DNA with T4 Polymerase - (reply: 6)
26. repeting PCR failing the reaction - (reply: 2)
27. Kanamycin resistance gene in Gram-positive bacteria - (reply: 2)
28. PCR fragment ligation - ligation of 3 pcr frangments (reply: 1)
29. Transformation Dilemma - I hope if you can figure out (reply: 5)
30. ligation and transformation problems - (wrong) oligos (reply: 4)
2. Got problem with maxiprep - (reply: 3)
3. selective preciptation of DNA according to size - (reply: 4)
4. dam methylation? - digestion problem (reply: 3)
5. how long does it take a colony inoculation to reach log phase? - (reply: 2)
6. Calculating transformation efficiency? - (reply: 1)
7. Calculating copy number? - (reply: 1)
8. Cloning into pGBKT7 vector - (reply: 4)
9. southern blotting problem with DIG - southern blotting problem with DIG (reply: 4)
10. pRARE2 vector sequence - (reply: 4)
11. Inverse PCR ligation - (reply: 1)
12. SapI enzyme - (reply: 3)
13. gene expresion - (reply: 1)
14. can you transform directly into liquid culture? - (reply: 2)
15. Is Pet22b from Novagen low copy? - (reply: 1)
16. ligation problem by using single RE - (reply: 8)
17. Plasmid DNA concentration - (reply: 2)
18. blunt-end ligation - (reply: 2)
19. False positives in clonation: long strain insert inside my colonies, please help - (reply: 1)
20. klenow/t4 and dNTPs - question (reply: 1)
21. Size of expressed Protein - (reply: 2)
22. DNA precipitation EtOH with Na OAc or Ammonium OAc - (reply: 4)
23. Phosphatasing to prevent vector reclosures - (reply: 1)
24. Troubleshooting! Strange growth in LB medium - (reply: 3)
25. Making blunt ends DNA with T4 Polymerase - (reply: 6)
26. repeting PCR failing the reaction - (reply: 2)
27. Kanamycin resistance gene in Gram-positive bacteria - (reply: 2)
28. PCR fragment ligation - ligation of 3 pcr frangments (reply: 1)
29. Transformation Dilemma - I hope if you can figure out (reply: 5)
30. ligation and transformation problems - (wrong) oligos (reply: 4)