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General-Lab-Techniques
1.
milky ethanol - DNA precipitation
(reply: 1)
2.
BSA is able to stop an enzymatic reaction? -
(reply: 1)
3.
How to solve Ammonium acetate -
(reply: 1)
4.
how to prepare phosphate buffer - 1M phospahate buffer
(reply: 1)
5.
dilutions again!please check - molar dilution
(reply: 2)
6.
concentration of primers -
(reply: 1)
7.
Gradient mixing -
(reply: 1)
8.
filtering and autoclaving solutions - anybody have any suggestions as to when and how to do it?
(reply: 3)
9.
Dilution -
(reply: 1)
10.
SDS vs. CTAB vs. Sarkosyl - cell lysis/DNA extraction
(reply: 6)
11.
.01 M Acetic Acid - How?
(reply: 4)
12.
Harvesting bacteria cells - Temperature of the centrifuge
(reply: 5)
13.
How to stop an enzimatic reaction -
(reply: 2)
14.
Collecting whole blood and plasma/serum - which tube to use, how to collect plasma/serum
(reply: 9)
15.
RNA wash: 75% EtOH or 100% EtOH? -
(reply: 1)
16.
Stability of IPTG, DTT at 4 degree -
(reply: 1)
17.
SDS-Page running -
(reply: 4)
18.
How to prepare buffer solutions? - I need some help here!
(reply: 3)
19.
Best storage temperature for samples - serum, protein, tissue, DNA, RNA...
(reply: 3)
20.
Quantifying Cell Lysis or the Presence of DNA -
(reply: 3)
21.
romanowsky staining -
(reply: 1)
22.
pre-analytical errors/factors - abnormal patient results
(reply: 4)
23.
Ferric Nitrilotracetate - How to make it out of FeCl3 and Na NTA
(reply: 1)
24.
SDS PAGE -
(reply: 3)
25.
what is the best way of releasing formaldehyde gas? -
(reply: 3)
26.
How to do agitation? -
(reply: 4)
27.
Autoclaving Glucose -
(reply: 5)
28.
0.2 M sodium acetate buffer at pH 3.0 -
(reply: 2)
29.
Phenol Extraction - Stupidity?
(reply: 1)
30.
EDTA and EGTA into solution - EDTA and EGTA into solution
(reply: 5)
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