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His-tag Related Discussions
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His-tagged protein not pure - (reply: 8)
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his-tag protein pure but degradated during growth - his-tag protein pure but degradated during growth (reply: 15)
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Problem with His Tag purification - using Promega HisLink spin purification kit (reply: 1)
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His-tag replaced with GST-tag! - (reply: 2)
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Purifying protein with no his tag - (reply: 5)
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help with His-tagged vector construct - (reply: 2)
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His-tag protein purification- buffers to use - What buffers to choose for His-tag chromatography (reply: 2)
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His tagged protein purification - (reply: 2)
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HIs tag recognition problem - Ways to expose the HIS tag (reply: 1)
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His tag fusion question - (reply: 2)
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Problem in purifying fusion protein with His Tag - Fusion protein found in Flow Through (reply: 6)
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How many His residues? - (reply: 1)
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His fused protein - (reply: 2)
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Elution of His-tag Protein under denaturing condition - (reply: 2)
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His-tag: C-terminal cleavable through protease? - (reply: 1)
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Protein in each elution step during His-Tag purification - (reply: 4)
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Name of double his tag vector - looking for name of company that sells the dual his tagged vector (reply: 3)
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HIS tag elution woes.. - (reply: 3)
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Purifying His-Tagged Proteins from Insects cells on IMAC resin? - (reply: 1)
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What's good and bad about C-tem and N-term His tag? - (reply: 3)
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Protein expression. Question of V5 epitope and 6x His tag ! - (reply: 2)
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His tag protein binding to resin ... - can i purify off the resin? (reply: 5)
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GST and His tagged proteins - Does the GST give problems in His tag purification? (reply: 5)
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His tag purification - competition with imidazole or histidine? (reply: 5)
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Protein isolation - Small size His-tag Protein isolation (reply: 4)
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pcDNA3.1 /V5 His Topo - (reply: 4)
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removal of his tag - (reply: 3)
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His-tag protein expression problem - (reply: 4)
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his-tag protein expression - inoculation of large cultures with plate colonies/ starter cultures vs. glycerol (reply: 1)
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his-tag thrombin cleavage in-column? - as a purification strategy (reply: 1)
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Purified protein (his-tagged) multiple bands on gel - all picked up by antibody - what could they be? (reply: 2)
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His tagged protein won't elute from nickel resin - (reply: 2)
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Anti-PolyHistidin antibody - Best His6 antibody for western (reply: 2)
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His tag purification - (reply: 1)
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His-Tag Question (pQE30/31/32 Qiagen Vector) - (reply: 2)
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HA tag vs His(6) tag , with mutagenesis - (reply: 6)
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His-tag protein is lost - (reply: 5)
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buffer for His-tag recombinant protein purification - (reply: 2)
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his-tag protein - (reply: 7)
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His Tag protein purification - (reply: 3)
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His-tagged proteins - bacteria or mammalian systems? - Which is better and why? (reply: 1)
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His tag protein purification problem - Binding of undesired protein (reply: 6)
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quick question about insoluble his-tag purification... - (reply: 2)
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His tagged protein from mammalian cells - purification of His tagged (reply: 1)
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His tagged antigen - Does the tag need to be removed? (reply: 3)
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His-tagged antigens - Must the tag be removed?? (reply: 1)
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His-tag fusion protein detection problem - (reply: 2)
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His tag cleavage by Thrombin - (reply: 2)
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Low yield at His tagged-peptide production in labeling media - (reply: 1)
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DNA amount for protein expression - protein His-tag (reply: 1)
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No expression of his-tagged protein - (reply: 4)
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problem purifying a his-tag protein - (reply: 2)
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Unexplained His-tag cleavage - (reply: 2)
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why get rid of imidazole in his tag protein purification - (reply: 3)
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lysis is not translucent after urea treatment - need help with His tag fusion protein purification (reply: 2)
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his tag protein not expressing - I am stuck !!! (reply: 6)
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6 His tagged protein purification - (reply: 1)
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His-tag design in primer - DO i need filler. (reply: 4)
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loss of His tag from protein made in mammalian cells? - Anyone have experience with His tag being cleaved off? (reply: 5)
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overexpress his-tag protein, wrong size?! - (reply: 1)
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His-tag protein purification - (reply: 1)
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problem in expression of his tag protein - (reply: 6)
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His tag detection problem - (reply: 2)
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HIS-TAG LIGATION - Ligation of His-tag into the pIMS vector (reply: 2)
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Silly question on His-tagged protein - (reply: 2)
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Appearence of non specific[non 6His tagged] proteins with Ni-NTA purification. - (reply: 16)
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His Tag purif buffer-to Tris or not to Tris? - best buffer for pH=7.0 (reply: 1)
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6 his tagged protein purification - purification (reply: 2)
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protein/enzyme activity - lost activity after his tagging (reply: 1)
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How can I reduce background in W blotting with polyclonal serum - from HIS-tagged fusion protein (reply: 3)
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Site directed mutagenesis - his tag - insertional mutations (reply: 6)
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small protein expression in yeast - expression of an 8kD his tagged protein in yeast (reply: 1)
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His-tag protein purificaton, cotamination with 40-43kD protein - (reply: 1)
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About pH and pI for his-tagged protein purification - (reply: 2)
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No expression of 7kDa His-tagged protein - why? - Poor protein expression (reply: 9)
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His-tag degraded after purification? - (reply: 3)
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His-tagged protein co-eluted with associated protein - (reply: 4)
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His6 tag quite problematic. - Is that true? (reply: 3)
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His Tag purification - specificity problem (reply: 8)
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can i autoclave buffers with imidazole for His-tag purification? - (reply: 2)
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His-tagged protein binding problem - Protein Binding (reply: 1)
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his tag - his tag purification (reply: 2)
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His-Tag purification using Qiagen Ni-NTA spin column - Extra band of unknown protein beside target protei (reply: 2)
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His-tag protein purification - Question (reply: 9)
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Protein with His6 tag - (reply: 2)
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Protein purification - Problem with his-tag protein gel filtration (reply: 5)
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How imidazole can be washed off the Ni-NTA agarose beads - reuse of Ni-NTA agarose beads for His protein (reply: 2)
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His-tag protein purification - contamination with other... - ...His-rich proteins (reply: 15)
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Problem with his tag protein purification - (reply: 2)
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his tag purification - (reply: 1)
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his tag removal - (reply: 6)
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his and myc tags - (reply: 1)
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His tag protein purification problem - (reply: 61)
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His tagged Flagellin purification - protein going into inclusion bodies (reply: 1)
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His purification of a regular expressed protein - (reply: 1)
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His-tag purification and his-antibody for western - (reply: 2)
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His-tag protein purification - (reply: 5)
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his-tagged protein purification - (reply: 1)
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What does his tag stand for ? - (reply: 1)
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Nickle column and His-tagged protein purification - recharging Ni+2 column (reply: 1)
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His tag - protein purification - (reply: 2)
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His-tag Westerns: Bestcommercial antibody - (reply: 1)