 plaque assay haven't worked |
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 Apr 9 2009, 01:49 PM
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member  Group: Active Members Posts: 17 Joined: 26-January 09 Member No.: 6426  |
hi Friends!!!
Everybody knows that Vero cells are commonly used in virology methods. I have tried for many times to make plaque assay with Vero cells but actually they are not working!!! in the past they did. i have other cells lines like LLC-MK2 and Hela and when the plaque assay are performed with these cells they are greatly good. My question: why vero cells have not been working in plaque assay? Thank you |
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Apr 9 2009, 11:34 PM
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 Metaller Scientist Group: Active Members Posts: 170 Joined: 30-January 09 Member No.: 6817 |
I'm very surprised to hear this, because I couldn't get plaque with HeLa so I decided to switch to Vero. Vero must work better I guess but it depends on the virus too. In the end I figure out not to have very confluent wells.
Is your virus a human virus or avian or..? This post has been edited by Curtis: Apr 9 2009, 11:35 PM |
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Apr 10 2009, 05:49 AM
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#3
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member Group: Active Members Posts: 17 Joined: 26-January 09 Member No.: 6426 |
QUOTE (Curtis @ Apr 9 2009, 11:34 PM)  I'm very surprised to hear this, because I couldn't get plaque with HeLa so I decided to switch to Vero. Vero must work better I guess but it depends on the virus too. In the end I figure out not to have very confluent wells. Is your virus a human virus or avian or..? Wow that's surprised too!! I work with Flavivirus, specially Dengue Virus and Yellow fever Virus. They frequently hit 105 PFU/ml Let me say:Is it 160 passage, Vero got old? thank you Reis,V.P. |
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