 How to make a tetracycline solution |
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 Mar 25 2005, 09:40 AM
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#1
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member  Group: Active Members Posts: 7 Joined: 23-February 05 Member No.: 5807  |
I am making at 5 mg/mL soln of Tetracycline, and am using EtOH per our most recent Molecular Cloning manual...but I am having trouble getting all of the solid dissolved. Should I be using 70 % ETOH? Any thoughts/suggestions would be appreciated!!
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Mar 25 2005, 12:56 PM
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#2
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member Group: Members Posts: 5 Joined: 22-March 05 From: Uruguay Member No.: 6195 |
Ive made some Tet sol. a couple of times....be sure to use an analytical balance, and dissolve it in absolute ethanol (100%)....and shake it up like theres no tomorrow...itll take a while but it will go into solution...
Do not autoclave...its ready for use.... Cheers QUOTE (slapolla @ Mar 25 2005, 10:40 AM) I am making at 5 mg/mL soln of Tetracycline, and am using EtOH per our most recent Molecular Cloning manual...but I am having trouble getting all of the solid dissolved. Should I be using 70 % ETOH? Any thoughts/suggestions would be appreciated!!
 -------------------- Mathias Casás Selves
Molecular Pathology Unit Dept. of Biochemsitry School of Medicine Montevideo, Uruguay |
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Mar 29 2005, 03:04 AM
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#3
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member Group: Active Members Posts: 19 Joined: 7-October 04 Member No.: 4308 |
i agree with matiascs. I do the same
chandima |
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Jan 29 2009, 05:54 AM
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#4
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 i'm unmethylated Group: Active Members Posts: 35 Joined: 29-January 09 Member No.: 6770 |
People,
Err... How do we know when to use water or ethanol? We have so much stocks here that I have yet to prepare any antibiotic solutions. -------------------- Too often we underestimate the power of a touch, a smile, a kind word, a listening ear, an honest compliment, or the smallest act of caring: all of which have the potential to turn a life around. ~ Leo Buscaglia
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Jan 29 2009, 07:53 AM
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#5
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 an elder Group: Active Members Posts: 555 Joined: 26-January 09 From: Staten Island, NY USA Member No.: 6470 |
QUOTE (dreamchaser_jc @ Jan 29 2009, 09:54 AM) People, Err... How do we know when to use water or ethanol? We have so much stocks here that I have yet to prepare any antibiotic solutions. you use that in which it is soluble and stable and will not interfere with your purpose. -------------------- talent does what it can
genius does what it must i do what i get paid to do |
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Jan 29 2009, 04:25 PM
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#6
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i'm unmethylated Group: Active Members Posts: 35 Joined: 29-January 09 Member No.: 6770 |
QUOTE (mdfenko @ Jan 30 2009, 12:53 AM) QUOTE (dreamchaser_jc @ Jan 29 2009, 09:54 AM) People, Err... How do we know when to use water or ethanol? We have so much stocks here that I have yet to prepare any antibiotic solutions. you use that in which it is soluble and stable and will not interfere with your purpose. Alright, got it! -------------------- Too often we underestimate the power of a touch, a smile, a kind word, a listening ear, an honest compliment, or the smallest act of caring: all of which have the potential to turn a life around. ~ Leo Buscaglia
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Feb 12 2009, 09:24 AM
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#7
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Veteran Group: Active Members Posts: 137 Joined: 27-January 09 Member No.: 6602 |
According to Lab-facts (Roche) make the stock in Et-OH and the dilutions in liquid culture.
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Feb 13 2009, 08:42 AM
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#8
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 Veteran Group: Moderator Posts: 441 Joined: 11-September 04 Member No.: 4036 |
I believe the confusion is that two forms of tetracycline are marketed, the compound, and its hydrochloride. Tetracycline is soluble in EtOH, but not water. Tet-HCl is soluble in water. Dissolving Tet-HCl in 50% EtOH is what we like, since it keeps the solution at -20C liquid.
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Feb 15 2009, 08:34 AM
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#9
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 Veteran Group: Active Members Posts: 138 Joined: 26-January 09 From: MY Member No.: 6530 |
QUOTE (phage434 @ Feb 13 2009, 08:42 AM) I believe the confusion is that two forms of tetracycline are marketed, the compound, and its hydrochloride. Tetracycline is soluble in EtOH, but not water. Tet-HCl is soluble in water. Dissolving Tet-HCl in 50% EtOH is what we like, since it keeps the solution at -20C liquid. And from my experience Tet-HCL is not as soluble in Ethanol compared to water. Tet-HCL in Absolute EtOH is quite crappy in term of solubility. This post has been edited by hanming86: Feb 15 2009, 08:34 AM -------------------- Lab + Coffee + Music = Bliss
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Mar 12 2009, 07:22 PM
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#10
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Enthusiast Group: Active Members Posts: 24 Joined: 19-February 09 From: Australia, Sydney Member No.: 7384 |
Hi Guys,
I f we make tetracycline stock solution i absolute or 100% ethanol instead of water, dont u think that ethanol is dangerous/killer for E.Coli cells? Yes i agree that not all tetracyline get dissolved , but i am still using it anyways by giving it a bit vortex. Please advise. Thanx. |
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Mar 12 2009, 08:34 PM
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#11
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Veteran Group: Moderator Posts: 441 Joined: 11-September 04 Member No.: 4036 |
The 1000x dilution you do when adding the stock to the medium makes the EtOH in the medium inconsequential.
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Mar 12 2009, 08:57 PM
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#12
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Enthusiast Group: Active Members Posts: 24 Joined: 19-February 09 From: Australia, Sydney Member No.: 7384 |
QUOTE (phage434 @ Mar 13 2009, 03:04 PM) The 1000x dilution you do when adding the stock to the medium makes the EtOH in the medium inconsequential. Thank you very much phage434. I did not know about it that we can do it. We have got Tet-Hcl and i would like to prepare it your way i.e 50% ethanol so it will remain in liquid form and no freeze thaw. Cheers |
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