 Best time for adding G418?, transfection and selecting |
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  Mar 12 2005, 06:13 AM
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#1
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member  Group: Active Members Posts: 7 Joined: 12-February 05 Member No.: 5663  |
hello everyone:
most protocol advice G418 was added 48h after transfection and some said 24h after transfection. I could see the obvious eGFP (the vector has eGFP gene) 12h after transfection. So, what is the best time to adding the G418? thanks in advance. |
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 Mar 13 2005, 06:35 AM
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#2
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Veteran Group: Active Members Posts: 291 Joined: 15-April 04 From: Bordeaux - France Member No.: 2987 |
hi
usually i wait 48h to ad G418 in a transfection asssay But when i make infections, 24h is ok (assuming your first infection is day 1 at about 10 and the G418addition is day 2 18...) that is ok for me. But it's only my advice. Maybe someone else has a better protocol. fred |
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Mar 14 2005, 03:33 AM
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#3
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Enthusiast Group: Active Members Posts: 95 Joined: 13-December 04 From: Barcelona Member No.: 5058 |
I usually add it 48 h after transfection, but I guess 24h should be as good.
Cheers! -------------------- Science is a wonderful thing if one does not have to earn one's living at it
(A.Einstein) |
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