efficiently transfect macrophage Options

[Daniel5306]

Does anybody know how to transfect macrophage? Now I am transfect J774A.1 and RAW264.7 with lipofectine from Invitrogen, but the transfection efficiency was very low. I want to know some method that can transfect macrophage efficiently and can get stable cell line. Any advice was appreciated.

Daniel From UNL

[erini]

you can use either retrovirus or adenovirus. Infection with retroviral vector will allow for stable expression of your gene, whereas adenoviral construct will ensure transient expression.

[wirly]

If you have access to an electroporator, electroporation works in almost every cell line but takes some monkeying to get the right conditions if you cannot find some already published. I have used is for cell lines that chemical tranfectants could not give stable transfections of.

I use a BTX unit and they have a lot of references to protocols for various cell lines. You can try some versions of their protocols.

http://www.btxonline.com/applications/prot...ian/Default.asp

good luck!