Help, macrophage transfection Options

[Daniel5306]

Does anybody know how to transfect macrophage? Now I am transfect J774A.1 and RAW264.7 with lipofectine from Invitrogen, but the transfection efficiency was very low. I want to know some method that can transfect macrophage efficiently and can get stable cell line. Any advice was appreciated.

Daniel From UNL

[mikew]

I've transfected U937 and Jurkat. I've only had success with electroporation.
In general, most suspension cells are resistant to transfection by any other technique.

[Daniel5306]

Thank your advice.
But the cells what I will use (J774A.1 and RAW264.7) were adhesive cell, which attach to the wall of the plate so firm that I have to use scraper when I subculture the cell. When I check the lipofectamine 2000 (invitrogen), the cell lines that can be transfected contained RAW264.7, but when I check the information of gencarrier 1/2, the information said they are better than lipofectamine. Some information indicated the Fugene6 might be better. It makes me fell difficult to decide which product I should try.
Thank you anyway!

Daniel from UNL

This post has been edited by Daniel5306: Dec 7 2004, 08:16 AM