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Sep 3 2004, 02:04 AM
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#1
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member ![]() Group: Members Posts: 4 Joined: 25-August 04 Member No.: 3883 |
HI
Can anyone suggest or offer advise on suitable markers for monocytes, macrophages and dendritic cells. I need to distinguish between the three cell types. I was thinking of using CD156a for monocytes, CD169 for macrophages and CD1a/DC LAMP for dentritic cells. Any ideas, comments would be most appreciated. Thank-you |
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Sep 16 2004, 08:23 AM
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#2
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member ![]() Group: Active Members Posts: 7 Joined: 15-September 04 From: Boston, MA. Member No.: 4075 |
For DC, you can use CD205 (DEC205). You can also use CD11c. For macrophages we typically use F4/80. If I remember correctly, you can double stain with CD14 and F4/80 for monocytes (hi CD14) and macrophages (low CD14). There is a great deal published in the area and you may want to do a PubMed search. The markers you chose will only identify subsets of each cell type.
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