 What is the mode of action of DMSO |
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 Apr 17 2004, 09:11 AM
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#1
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member  Group: Members Posts: 2 Joined: 16-April 04 Member No.: 2995  |
hey all
Iam a new user here and I have a question What is the mode of action of DMSO and glycerol as preservatives during the freezing of the cells Thanks in advance |
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 Apr 17 2004, 05:10 PM
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#2
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Enthusiast Group: Active Members Posts: 90 Joined: 1-April 04 From: CO, USA Member No.: 2901 |
DMSO prevents oxygen interaction with the liquid meniscus and also keeps the cells in a transformation efficient state when dealing with competent cells possibly by keeping the lipid layers distended. Glycerol prevents ice formation that would otherwise rupture delicate membranes-acts like an automobile antifreeze. Mycobacteria are the only bacterial cells that donot need glycerol for storage-infact glycerol is detrimental for use there.
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May 27 2004, 04:34 AM
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#3
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member Group: Active Members Posts: 18 Joined: 3-February 04 From: Belarus Republic Member No.: 2660 |
Glycerol and DMSO prevents formation of big cristalls of water, that breakdown membrane. Also as the media begins to
freeze the salt concentration outside the cells will become greater than that in the cells and water will leave the cells to be replaced by the DMSO or glycerol. This post has been edited by Shubenok: May 27 2004, 05:32 AM |
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