 Macrophage detached |
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 Apr 3 2001, 09:00 PM
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#1
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Veteran  Group: Posts: 1891 Joined: -- Member No.: 1  |
Dear SirI am currently using macrophages (5-7 days old) to run flowcytometery on these cells. The first step to start with is to detach cells from plate/flask. Would you please tell me what is the best way to detach these cells without any disturbance to the cell surfceusually I am using cold 0.02% of EDTA and 0.25% trypsine and still I have difficulties to lift these cells up in short time beside lose almost 30% of cells.Many thanks for your help.
M. AlaliCentre for Virus ResearchNSW, Australia |
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Apr 25 2001, 09:00 PM
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#2
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Veteran Group: Posts: 1891 Joined: -- Member No.: 1 |
To detach macrophages you could use lidocaine (4 mg/ml) togetherwith 5 mM EDTA. leave them at 37C for 3-5 minutes and they should come off.
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