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> Macrophage culture
Sinead
post Jun 8 2002, 05:41 AM
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I am trying to culture macrophages from whole blood. What I am looking for is a way to assess the purity of my macrophage population adhered to the culture dish.
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RichardColes
post Jun 27 2002, 05:56 PM
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Use Mac-1 and GR-1(granulocytes) antibodies and do FACS analysis or something like that.
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febstein
post Nov 24 2003, 03:43 AM
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Alternatively, have you ever tried to generate macrophages from monocytes in the presence of GM-CSF ?
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Victor
post Dec 19 2003, 02:45 PM
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Can also try diI labeled acetylated LDL to test their phagocytic activity.
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rakeshbhu
post Nov 29 2004, 07:24 AM
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Time of adheration and washing of adhered monocytes are very important. Standardized it.
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