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> Cycloheximide Concentration
mattbat7
post Oct 7 2009, 07:23 AM
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Hi,

I'm trying to inhibit a stock strain of wild-type Saccharomyces with cycloheximide.....but am unsure what concentration to use.


Does anybody know of a resource i can use, or what concentration is commonly used?

Thanks

Mattbat
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GeorgeWolff
post Oct 7 2009, 01:38 PM
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Matt - the levels I've used are ~ 0.5g/liter.
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GeorgeWolff
post Oct 7 2009, 03:31 PM
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Saw (old) paper (http://jb.asm.org/cgi/content/abstract/86/5/956 ) reporting apparent cycloheximide induction of petite variant of related species.
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