What Carbenecillin concentration does everyone normally use??? (View forum version)



Ms new scientist

Posted 24 August 2009 - 10:12 AM

Hi Guys

I was just wondering what is the typical carbenecillin concentration used in your labs for making agar plates and such. I use 50micrograms per ml. Is that too little?

thanks for your help.

HomeBrew

Posted 24 August 2009 - 12:25 PM

When I worked in Pseudomonas, we used to use 50 ug/ml for E. coli, and 300 ug/ml for Pseudomonas aeruginosa.

labtek

Posted 24 August 2009 - 06:06 PM

Sigma suggests 100 ug/ml for their shRNA bacterial culture.

T C

Posted 25 August 2009 - 04:21 AM

Hey,

I too use 50 ug/ml for E. coli.

Best,
TC

Hi Guys

I was just wondering what is the typical carbenecillin concentration used in your labs for making agar plates and such. I use 50micrograms per ml. Is that too little?

thanks for your help.