ELISA Help-stopping (View forum version)



tholusc

Posted 20 July 2009 - 09:41 AM

Is it possible to stop an ELISA (HRP used as enzyme, OPD/H2O2 as the substrate) with concentrated HCL? I see many protocols calling for stopping with H2SO4, will there be any difference in OD values since both HCL and H2SO4 will make the solution very acidic?

Thanks!

Gerard

Posted 20 July 2009 - 11:13 AM

Yes you can, I've used both in different elisa's en didn't notice a difference.
Don't use concentrated HCl but 1 or 2 M HCL