sequencing result for primer designing (View forum version)



hazrina

Posted 17 June 2009 - 01:19 AM

how to know that our sequence hit the gene not the primer?im so confused....

HomeBrew

Posted 17 June 2009 - 03:49 AM

Wlecome to the BioForum, hazrina!

I'm not sure what you're asking... Did you obtain some sequence data using a sequencing primer? If so, the sequence will begin (theoretically) at a point one base 3' to where the primer binds. You usually can't read the first 10-20 bases of the sequence, so the sequence proper would begin some short distance away from the 3' end of the primer.

If you re-ask your question with more detail about what you did and what you're confused about, I'm sure we can be of more help...