Out of stock: trimethyl H3K4 antibody for ChIP (View forum version)



jiro_killua

Posted 18 April 2009 - 05:42 PM

Abcam is out of H3K4me3 antibody (ab8580) again, and this time quite seriously

It was out of stock for some time now, and they initially claimed it will be available in March

But the batch in March didn't pass their quality control, and they now announced it won't be availble until Sept

That's a blow to whoever uses ab8580 to do ChIP in mouse tissues/cells

So I'm asking if anyone had tried any replacement/substitute of ab8580 and had success?

I don't want to sit and wait until Sept

KPDE

Posted 20 April 2009 - 01:38 PM

Abcam is out of H3K4me3 antibody (ab8580) again, and this time quite seriously

It was out of stock for some time now, and they initially claimed it will be available in March

But the batch in March didn't pass their quality control, and they now announced it won't be availble until Sept

That's a blow to whoever uses ab8580 to do ChIP in mouse tissues/cells

So I'm asking if anyone had tried any replacement/substitute of ab8580 and had success?

I don't want to sit and wait until Sept


We tried a H3K4m3 antibody from Diagenode (cat# pAB MEHAHS-024) in our 96-well plate based ChIP assay and it worked great (we got about 30-50% of input; the % of input is much higher for our plate based assay than traditional ChIP so it's hard to compare this recovery to other protocols). I never compared the Abcam and Diagenode antibodies in our plate based assay so I can't say how comparable they are.

Davo

Posted 22 April 2009 - 05:09 PM

I used the an anti-H3K4me3 antibody from Upstate and I got a good level of pull down with it.

lipid

Posted 22 April 2009 - 09:58 PM

I used the an anti-H3K4me3 antibody from Upstate and I got a good level of pull down with it.


Thank you for your information (I also have been looking for a good antibody for ChIP).
Did you mean MC315?
http://www.millipore...gue/item/04-745

or AW304?
http://www.millipore...gue/item/04-791

Thank you in advance.

best regards,
lipid

Davo

Posted 03 May 2009 - 05:27 PM

The catalogue number when I ordered it was 05-745. The labelling says it was MC315.

I wish you luck!
David

lipid

Posted 06 May 2009 - 08:03 PM

The catalogue number when I ordered it was 05-745. The labelling says it was MC315.

I wish you luck!
David


Thank you very much for your valuable information!

Best,
lipid