poly-D-lysine coated covr glsses and HeLa cells (View forum version)



Keshet

Posted 01 April 2009 - 12:40 AM

well, this is my first question here... :angry:

i have a little problem,
whenever i seed Hela cells on poly-d-lysine coated cover glasses, they dont sit well, compering to the plate.
i dont have this problem with HEK293.
any advise?


thanks!
keshet. :D

bob1

Posted 02 April 2009 - 03:32 PM

HeLa don't have very strong attachment, try leaving them for 36-48 hours. You could try another coating- gelatine works well usually.