plasmid spin column usage (View forum version)



shimshady

Posted 16 February 2009 - 12:54 PM

I ran out of the PCR clean up, spin through columns. Are the plasmid miniprep spin through columns the same thing? Are there any other protocols i can use that do not utilize the columns if they are not the same. Thanks

Drew

phage434

Posted 16 February 2009 - 02:03 PM

They are the same.

bob1

Posted 16 February 2009 - 02:42 PM

They are similar, often the membranes on them are specifically designed with molecular weight cut-offs (higher and lower MW), so be careful about using plasmid ones with small PCR fragments.

perneseblue

Posted 16 February 2009 - 04:05 PM

note, you can easily reuse the spin columns. We have a very useful article on this here.

It does work.

T C

Posted 16 February 2009 - 07:52 PM

Hey

Like people have said, it can be used. However, if you don't want to use the columns, you can always precipitate the DNA; the yields are generally higher but it takes more time.

TC

molgen

Posted 18 February 2009 - 03:01 AM

Try glycogen precipitation