RNA Extraction & EMSA (View forum version)



Mayflower

Posted 15 February 2009 - 10:57 AM



Hi,

I want to extract RNA from tissue culture cells for EMSA can anybody describe (or outline the steps) in brief the procedure from extraction to EMSA?

molgen

Posted 16 February 2009 - 02:53 AM

I think that any extraction will do.
Once you have the RNA you have to mix in the protein and run it on a gel to see the shift.