Dilution of antibody (View forum version)

Rojan D.

Posted 31 July 2018 - 01:10 AM

hi everyone. 


im a newbie in the lab and I've ordered a new antibody that recommend to use the diluton 1µg/ml. 

The vial is 100µl of the antibody. However, I do not know the concentration within the 100µl. 


Can anybody help me to resolve my question which is, what is 1µg/ml in terms of dilution factors such as 1:1000 etc. 




Posted 31 July 2018 - 05:49 AM

Check the data sheet that came with the antibody and have a look on the tube too. If you can post a link to the antibody that might help


Antibodies commonly come as 1 mg/ml and 0.5 mg/ml concentrations, though other concentrations are also supplied, so don't assume either of these.