Protein concetation at 280 nm (View forum version)



samita

Posted 09 September 2015 - 04:52 AM

Any nice and simple protocol to determine the protein concentation at 280 nm if the extinction coefficient is known..

 

 


labtastic

Posted 10 September 2015 - 11:52 AM

Measure A280...dilute sample if necessary to ensure sample reading is between 0.1 and 0.5.  

 

Never trust an absorbance reading above 1.0.

 

Exception: nano-drop specs can read much higher absorbances. Dyanamic range is ~1-100 for nanodrops.

 

Then, Beer's law.