Diluted Sample OD (View forum version)



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Posted 06 August 2013 - 11:12 PM

Undiluted Solution has OD of >1 and after diluting two times OD is 0.6. how to calculate OD of undiluted Sample?

 

 

Is it 0.6 X 2 = 1.2 ? ( Multiplied OD by Dilution Factor)

 

thanks.


pcrman

Posted 06 August 2013 - 11:30 PM

I think your original OD should be 2.4 assuming your dilution is 1:1.  After first dilution, you get 1.2, and 0.6 the 2nd time. 

 

the formula is 0.6/0.5/0.5 = 2.4


newborn

Posted 07 August 2013 - 05:19 AM

May be he meaned that he diluted it at 1:2. if that was the case 1.2 was correct.


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Posted 08 August 2013 - 12:02 PM

Thank you both. I did diluted 1:2