Digestion of pRSETA vector leads to funny migration profile (View forum version)



Gautam

Posted 12 June 2013 - 03:08 AM

I am running agarose gels of Eco RI digested pRSET A but the cut vector shows lower mobility than the uncut
Is this a problem specfic for pRSET ?

  • ex 47  bk 3 p120 Eco RI prSET 2U.jpg

scolix

Posted 12 June 2013 - 04:27 AM

coiled DNA (uncut) runs faster than linear DNA.

Gautam

Posted 12 June 2013 - 08:39 PM

Yes I am aware of that-but I am not able to figure this one out-the cut band shows the expected soze of 2.9 kb but i wonder if it is Star activity of EcoRI or something else -like nuclease contamination somwhere-now trying out with other cutters of pRSET