crosslinking of protein and generate antibody (View forum version)



neuron

Posted 16 January 2013 - 11:36 PM

Has anyone tried to crosslink 3-4 proteins and inject in mice or rabbit?

Curtis

Posted 19 January 2013 - 09:33 AM

what is the use of the generated antibody? I'm curious. Does it bind to all the proteins?

neuron

Posted 19 January 2013 - 10:40 PM

I also don't know. It should not bindPosted Image . I was wondering if somebody has tried generating antibody against a complex. I asked this question in another thread also-

http://www.protocol-...rotein-complex/

As chelo mentioned that it has been done, I dint find any publication on that. And as crosslinkers are available to capture the protein interactions. I was thinking is it possible to generate antibodies for two proteins by crosslinking them?Posted Image