values for mRNA molecules per cell (View forum version)



madison-twadder

Posted 03 May 2012 - 05:08 AM

Hi folks,

I am looking for reliable numbers of abundane of mRNA species in human cells (in molecules per cell or M), or at least a rough estimates like for a housekeeping gene / a typical transcription factor the numer is x / cell.

I guess the concentration also varies largely and depends on different factors like cell volume variation, cell cycle etc. Can you recommend papers on that?

Thanks a lot,
Mad

bob1

Posted 03 May 2012 - 01:16 PM

Roche Lab FAQs suggest that the total RNA per cell is about 100 fg and the proportion of mRNA is 1-5%.