OVERDILUTION OF TBE BUFFER (View forum version)



helencollins560

Posted 01 May 2012 - 03:11 PM

HI ALL

I wanted to make a 1x TBE solution from a 1 Litre bottle of Lonza Accugene 10x TBE buffer. However, instead of adding 9 Litres of milliq water to the buffer I added 10-11 litres of milliq water.

I wanted to know two things

[a] can i use this TBE as it is to make DNA gels for genotyping

or

[b] could i just get another bottle of 10x TBE add it to my existing stock then bring up to 20 litres with milliq to even out my error


thanks

phage434

Posted 01 May 2012 - 05:05 PM

It will probably work as is. You should make your gels with the same buffer.

You can fix this as you suggest by making additional buffer, if you would like.