Reuse of SDS-PAGE electrode buffers (View forum version)



Glyoxime

Posted 15 March 2012 - 12:26 PM

Hi everyone:

I have always heard that the anode buffer (outer chamber in Miniprotean 3) can be reused many times but the cathode buffer (inner chamber) must always be fresh. Is this true? If so, why?

Thanks!!

Curtis

Posted 15 March 2012 - 07:38 PM

Biorad's? I always use just one tank buffer for Mini Protean 3 and re-use multiple times until it turns cloudy.

mdfenko

Posted 16 March 2012 - 11:44 AM

because the cathode buffer will be depleted.