inquiry about the methods for gene knockout in bacteria (View forum version)


Posted 13 March 2012 - 01:28 PM

Hi I want to knockout a gene in a bacteria.

I am a new starter. Would you please give a general picture about how many methods I can choose, so that I can read more about them and choose a suitable one? I get confused when I read papers for their different methods, so I want to get a big picture first.

Thanks a lot! And look forward for your response!


Posted 02 August 2012 - 03:13 AM

Its just flanking region of gene you have to choose. goal is to add resistant marker replacing middle portion of flank, then later removing resistant marker too.
- thats all you have mutant.