Basis/Protocols for Choosing concentrations in testing medicinal effect plant ex (View forum version)



chowee

Posted 31 January 2012 - 05:57 AM

I was conducting a study to test a medicinal effect of a specific plant..I did not see any protocols on what concentration should i used. I decided to use 25%,50%,75% and 100% of the extract but still i should have a basis for using it..Anyone can help me on this...thank you so much..

scolix

Posted 31 January 2012 - 06:40 AM

I guess you would like to use a compound at a concentration comparable to its affinity or better to its EC50 to its intended target protein.

akhshik

Posted 03 February 2012 - 10:05 PM

it is really depend on the method of extraction is it a simple infusion or a super critical extraction method? bellow you could find a useful guide:

http://www.unido.org...atic_plants.pdf

Cheers,
Akhshik.