problem with embryo carcinoma cell line , p19 , (View forum version)



ohoud

Posted 01 September 2011 - 03:04 AM

problem with embryo carcinoma cell line , p19 ,


30 August 2011 - 12:39 PM


hi

i am phd student

i am working on p19 cell lines
in the beginning of my first year the p19 cell line was supper confluent nearly 80-90% within 24hr and they were in a good condition
however, nowadays i have problem growing them. they start growing very slowly and they detached from the flask easily.
i have not change the medium alphaMEM with boven calf serum 7.5% and FBS 2.5%

i do not know why they start behave this way

any recommendation or opinion about what may go wrong with p19 cell line

thank you in advance
ohoud


GNANA

Posted 01 September 2011 - 11:32 PM

have you done a recent check for mycoplasma???