Sybr Green Real Time PCR - Amplification plot (View forum version)



siuchi98

Posted 18 May 2011 - 12:38 AM

Dear all,

Here are my Sybr Green Real Time PCR amplification plots. May I ask if there is any problem?

Thank you so much.

siuchi

  • Amplification Plot 1.jpg
  • Amplification Plot 2.jpg
  • Amplification Plot 3.jpg
  • Amplification Plot 4.jpg
  • Amplification Plot 5.jpg
  • Amplification Plot 6.jpg
  • Amplification Plot 7.jpg

Trof

Posted 18 May 2011 - 02:08 AM

These are amplification curves in log view, if you want to look on the curves you better use the linear view, you can't see anything from this.
Also, why do you ask, what problem do you expect? There isn't much to tell even from linear chart. If you have doubts about your results you better check the melting curve to see if you have a single product.