Micropipetting technique (View forum version)



shyamadi

Posted 17 May 2011 - 12:18 AM

How can we get the at most accuracy while using pipetting ??? Though I use pipettes since many years but i always find some inaccuracies despite of calibration.

mdfenko

Posted 17 May 2011 - 07:06 AM

read and follow the literature that came with the pipet and practice, practice, practice.

hobglobin

Posted 17 May 2011 - 09:02 AM

using a balance to control pipetting was suggested several times before

here is a powerpoint presentation from Rainin with several hints and some good advice:

 raininpipettetechniques.ppt   1.83MB   5810

shyamadi

Posted 18 May 2011 - 01:14 AM

Dear mdfenko,
Many many thanks and yeah I do agree there is no way besides practicing.

shyamadi

Posted 18 May 2011 - 01:17 AM

Dear hobglobin,
That was really valuable piece of information and I am hoping to resolve my simplest errors. Millions of Thank.