Compare ChIP to cell lysate (View forum version)



mcb56

Posted 15 April 2011 - 04:14 AM

I am doing a chromatin immunoprecipitation and I would like to compare the amount of protein I purify with my antibody to the amount of the same protein left over in my cell lysate. I can't use a loading control because the ChIP will only have my protein of interest and nothing else to compare it to.

Thank You

ssmemo

Posted 01 December 2011 - 02:19 PM

Did you figure out what type of control was appropriate? I'm curious too.