Wet transfer vs Dry transfer (View forum version)



neuron

Posted 29 March 2011 - 02:57 AM

Hi,

I have done western blots for 2-3 times and only by wet transfer. My protein size is between 15-17 Kda. We have only Semidry transfer apparatus available in lab, I borrowed wet transfer apparatus from other lab. Since now I've to western blotting regularly :( , I can't borrow the apparatus always......i asked people about semidry apparatus but everybody says that for small protein wet transfer is better....Is that true?? :unsure: ..

mdfenko

Posted 29 March 2011 - 08:32 AM

i find that i get good transfer of small proteins with semi-dry apparatus and 0.2um pore membrane.

knuf

Posted 30 March 2011 - 08:04 AM

We were looking at a dry transfer apparatus and found that it worked fine for small proteins, but much less well for large proteins--but this was dry vs wet, not semi-dry. But I would think that it would be similar.