New to Gene cloning (View forum version)



SciCell

Posted 10 March 2011 - 10:21 AM

Hello all,

I am a cell culturist and completely new to Molecular biology stuff. All I do is get the plasmid vector / DNA / virus from another group, do transfections, grow the cells, do selection, analyze the cells for expression using various cell based assays, FACS, clone and develop stable cell lines expressing recombinant protein.
But I have never done the plasmid vector generation and would like to do some of that part in my job. I don't want to ask my boss looking like a fool. I know this is basic to molecular biology. Can you suggest a broad methodology / web links on how I can get started? I know there is lot of info on the web - which is confusing to me.
I am not completely naive to the subject - cause I've done DNA purification, restriction digest, running the digests and doing southern blotting in my old job.

Thanks for your replies.

pDNA

Posted 10 March 2011 - 11:28 AM

you can go for the Qiagen Bench Guide ...very useful for your purpose!

Regards,
p

Hello all,

I am a cell culturist and completely new to Molecular biology stuff. All I do is get the plasmid vector / DNA / virus from another group, do transfections, grow the cells, do selection, analyze the cells for expression using various cell based assays, FACS, clone and develop stable cell lines expressing recombinant protein.
But I have never done the plasmid vector generation and would like to do some of that part in my job. I don't want to ask my boss looking like a fool. I know this is basic to molecular biology. Can you suggest a broad methodology / web links on how I can get started? I know there is lot of info on the web - which is confusing to me.
I am not completely naive to the subject - cause I've done DNA purification, restriction digest, running the digests and doing southern blotting in my old job.

Thanks for your replies.


SciCell

Posted 11 March 2011 - 03:49 PM

Thank you for your reply. Yes, it is useful. Refreshing my memory what I did at school some time back.

Regards,

you can go for the Qiagen Bench Guide ...very useful for your purpose!

Regards,
p


Hello all,

I am a cell culturist and completely new to Molecular biology stuff. All I do is get the plasmid vector / DNA / virus from another group, do transfections, grow the cells, do selection, analyze the cells for expression using various cell based assays, FACS, clone and develop stable cell lines expressing recombinant protein.
But I have never done the plasmid vector generation and would like to do some of that part in my job. I don't want to ask my boss looking like a fool. I know this is basic to molecular biology. Can you suggest a broad methodology / web links on how I can get started? I know there is lot of info on the web - which is confusing to me.
I am not completely naive to the subject - cause I've done DNA purification, restriction digest, running the digests and doing southern blotting in my old job.

Thanks for your replies.