Dissolved Primer pellet in pure ethanol instead of water (View forum version)



Moebius

Posted 09 March 2011 - 06:49 AM

I accidentally grabbed the ethanol bottle instead of the water one. The ethanol bottle has 100% ethanol. I noticed the stock solution didn't freeze at -20 C and I didn't get any results for my PCR reaction. I was wondering if I can just pipette out the ethanol and add the appropriate amount of water into the primer tubes. I can still see the pellet, but some of the pellet has gotten bigger in area. I really appreciate any help in advance! Thank you!

Rsm

Posted 09 March 2011 - 10:18 AM

Spin down, decant, air dry, resuspend, and don't post in all forums at the same time.

Ameya P

Posted 09 March 2011 - 09:50 PM

Is that how he got his 7 posts???? :P

Rsm

Posted 10 March 2011 - 04:55 AM

Is that how he got his 7 posts???? :P


What do you think how I got my posts? Ctrl+c Ctrl+v !!! :lol:

Ameya P

Posted 10 March 2011 - 04:58 AM


Is that how he got his 7 posts???? :P


What do you think how I got my posts? Ctrl+c Ctrl+v !!! :lol:


I'm catching up.... :P