western without tween (View forum version)



nataly

Posted 21 February 2011 - 12:15 AM

hi,
I forgut to put tween while doing the western blot. will it change the results drastically?

BioMiha

Posted 21 February 2011 - 12:42 AM

Not really. You could observe slightly more non-specific binding but I wouldn't worry about it too much.

little mouse

Posted 21 February 2011 - 12:44 AM

hi,
I forgut to put tween while doing the western blot. will it change the results drastically?


you might have more background and/or non specific bands, or not, depending on your antibody.
good luck

nataly

Posted 21 February 2011 - 01:04 PM

thanks