Differences in PBS (View forum version)



EstherEsther

Posted 07 January 2011 - 01:39 AM

Why do all PBS contents differ from one and other. :wacko:
Maniatis, Lonza, R&D, all PBS contents differ. Why is this, what is the difference and what should I use?! (In my case for an ELISA wash buffer)

Curtis

Posted 07 January 2011 - 02:01 AM

yes, it's true, but don't forget the most important factor is the pH.

sgt4boston

Posted 07 January 2011 - 03:28 AM

For Elisa wash buffer Sigma PBS or any buffered saline solution is fine pH7.4. You may also add in small amount of BSA. Read some of the entries in the immunoassay/immunology section. Number of washes, inclusion of surfactant, time left in wells are some other variables.

EstherEsther

Posted 07 January 2011 - 03:52 AM

Thanks.
Still weird that the concentrations of the contents can be so different.