question about peroxidase activity calculation (View forum version)



iraj

Posted 07 December 2010 - 09:32 PM

hi all
i am working about plant peroxidase activity.
after determination of peroxidase activity by spectrophotometer
i dont know what i calculate peroxidase activity.
for enzyme activity i used buffer phosphate and enzyme and hydrogen peroxide in 460 nm.

thanks

protolder

Posted 09 December 2010 - 11:17 PM

Hola, In the descrption of the spectrophotometric method you have to have the relation betwen ud enzyme/ O.D., But the most appropiate is doing your own standar curve with definited amounts of pure protein ,or with known specific activity and compare with yours.Commercial horseradish perosidase have different units/mg depending if the valoration is made with pirogallol or ABTS. Make a graph with amounts of commercial peroxidase against O.D. and the yours Relationing the responses of both you can know how pure is your preparation. Buena suerte