why only SDS (View forum version)



anuj

Posted 09 September 2010 - 06:08 AM

why do we use only SDS in PAGE?? why cant it be some other detergent like butanyl sulfate??

mdfenko

Posted 09 September 2010 - 06:44 AM

sds was found to be highly efficient in solubilizing and dissociating protein complexes. that and its ability to part a (roughly) equivalent charge density to the proteins makes it useful for separations based on size.

anuj

Posted 09 September 2010 - 10:29 PM

sds was found to be highly efficient in solubilizing and dissociating protein complexes. that and its ability to part a (roughly) equivalent charge density to the proteins makes it useful for separations based on size.


thnx for ur reply...bt m sory to say i dont find it to be satisfactory ans..

mdfenko

Posted 10 September 2010 - 07:12 AM

the long hydrophobic tail wraps around the hydrophobic parts of the protein.

for more, see these references (i don't have access):

shapiro et al, bbrc (1967)

sds page, encyclopedia of immunology

one dimensional sds page, encyclopedia of separation science