thiourea in 2D page (View forum version)



shmaggregates

Posted 15 July 2010 - 10:39 AM

Is the thiourea included to scavenge excess alkylating agent prior to running the 2nd dimension of 2D gels?

braincow

Posted 19 July 2010 - 08:30 AM

While I suppose thiourea can react with IAA, excess IAA probably doesn't hinder PAGE. I'm not sure that it's known what exactly thiourea does, biochemically speaking, in 2D PAGE.